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Colloidal gold nanoparticle conjugates of gefitinib

Title
Colloidal gold nanoparticle conjugates of gefitinib
Author
주재범
Keywords
Lung cancer cells; Gold nanoparticle conjugates; Gefitinib; Cell viability; Surface-enhanced Raman scattering
Issue Date
2014-11
Publisher
ELSEVIER SCIENCE BV
Citation
COLLOIDS AND SURFACES B-BIOINTERFACES, 권: 123, 페이지: 61-67
Abstract
Gefitinib (GF) is a US Food and Drug Administration-approved epidermal growth factor receptor (EGFR)tyrosine kinase inhibitor for treating the lung cancers. We fabricated colloidal gold nanoparticle (AuNP) conjugates of the GF anticancer drug by self-assembly to test their potency against A549, NCI-H460, and NCI-H1975 lung cancer cells. GF adsorption on AuNP surfaces was examined by UV vis absorption spectra and surface-enhanced Raman scattering. Density functional theory calculations were performed to estimate the energetic stabilities of the drug-AuNP composites. The N1 nitrogen atom of the quinazoline ring of GF was calculated to be more stable than the N3 in binding Au cluster atoms. The internalizations of GF-coated AuNPs were examined by transmission electron and dark-field microscopy. A cell viability test of AuNP GF conjugates with the EGFR antibody exhibited much higher reductions than free GF for A549, NCI-H460, and NCI-H1975 lung cancer cells after treatment for 48 h. (C) 2014 Elsevier B.V. All rights reserved.
URI
https://www.sciencedirect.com/science/article/pii/S0927776514004445?via%3Dihubhttp://hdl.handle.net/20.500.11754/51465
ISSN
0927-7765; 1873-4367
DOI
10.1016/j.colsurfb.2014.08.021
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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