Oligopeptide complex for targeted non-viral gene delivery to adipocytes
- Title
- Oligopeptide complex for targeted non-viral gene delivery to adipocytes
- Author
- 김용희
- Keywords
- CURRENT STRATEGIES; ANTIOBESITY DRUGS; ADIPOSE-TISSUE; SIRNA DELIVERY; NORMAL RATS; THERAPY; OBESITY; INFLAMMATION; PROHIBITIN; ADIPOKINES
- Issue Date
- 2014-10
- Publisher
- 한국고분자학회
- Citation
- NATURE MATERIALS, 13권, 12호, pp. 1157-1164
- Abstract
- Commercial anti-obesity drugs acting in the gastrointestinal tract or the central nervous system have been shown to have limited effcacy and severe side effects. Anti-obesity drug development is thus focusing on targeting adipocytes that store excess fat. Here, we show that an adipocyte-targeting fusion-oligopeptide gene carrier consisting of an adipocyte-targeting sequence and 9-arginine (ATS-9R) selectively transfects mature adipocytes by binding to prohibitin. Injection of ATS-9R into obese mice confirmed specific binding of ATS-9R to fat vasculature, internalization and gene expression in adipocytes. We also constructed a short-hairpin RNA (shRNA) for silencing fatty-acid-binding protein 4 (shFABP4), a key lipid chaperone in fatty-acid uptake and lipid storage in adipocytes. Treatment of obese mice with ATS-9R/shFABP4 led to metabolic recovery and body-weight reduction (>20%). The ATS-9R/shFABP4 oligopeptide complex could prove to be a safe therapeutic approach to regress and treat obesity as well as obesity-induced metabolic syndromes.
- URI
- http://dx.doi.org/10.1038/NMAT4092http://hdl.handle.net/20.500.11754/51040
- ISBN
- 1476-4660
- ISSN
- 1476-1122
- DOI
- 10.1038/NMAT4092
- Appears in Collections:
- COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
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