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Use of surface-enhanced Raman scattering to quantify EGFR markers uninhibited by cetuximab antibodies

Title
Use of surface-enhanced Raman scattering to quantify EGFR markers uninhibited by cetuximab antibodies
Author
주재범
Keywords
SERS; EGFR; Antibody drug; SERS imaging; Cetuximab; GROWTH-FACTOR RECEPTOR; PATTERNED MICROARRAY CHIP; HOLLOW GOLD NANOSPHERES; BREAST-CANCER; SINGLE-MOLECULE; SERS; CELLS; IMMUNOASSAY; FABRICATION; SIZE
Issue Date
2014-10
Publisher
Elsevier Science B.V., Amsterdam.
Citation
BIOSENSORS & BIOELECTRONICS, 60권, pp.358-365
Abstract
Epidermal growth factor receptor (EGFR) has been recognized as an important prognostic marker expressed in cancer cells because its activation is associated with key features of cancer including tumor growth, survival, angiogenesis, and metastasis. Cetuximab is the first monoclonal antibody drug that targets EGFR overexpressed in cancer cells. It easily binds to EGFR, thereby down-regulating the receptor, blocking EGFR-mediated tyrosine kinase activity, and inhibiting cellular proliferation. Thus, EGFR-cetuximab binding can be quantified to monitor receptor status and the prognosis of cancer therapy. In this work, we report using SERS imaging to assess the inhibitory effect of cetuximab on EGFR expressed on cancer cells. From SERS mapping images using silica-encapsulated gold nanotags, the localized spatial distribution of EGFR that was not inhibited by cetuximab could be determined. Furthermore, EGFR expression could be accurately quantified through the statistical analysis of surface-enhanced Raman scattering (SERS) spectral data. Our experimental data demonstrate the feasibility of SERS imaging to improve the prognostic efficacy of cetuximab treatment. (C) 2014 Elsevier B.V. All rights reserved.
URI
http://dx.doi.org/10.1016/j.bios.2014.04.041http://hdl.handle.net/20.500.11754/50542
ISBN
1873-4235
ISSN
0956-5663
DOI
10.1016/j.bios.2014.04.041
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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