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A Liquid Chromatography-Tandem Mass Spectrometry Method for Simultaneous Quantitation of 10 Bioactive Components in Rhus verniciflua Extracts

Title
A Liquid Chromatography-Tandem Mass Spectrometry Method for Simultaneous Quantitation of 10 Bioactive Components in Rhus verniciflua Extracts
Author
유혜현
Keywords
STOKES EXTRACT; CELL-LINES; QUALITY
Issue Date
2016-03
Publisher
OXFORD UNIV PRESS INC
Citation
JOURNAL OF CHROMATOGRAPHIC SCIENCE, v. 54, No. 3, Page. 390-396
Abstract
The purpose of this study was to develop a simultaneous method to quantify 10 bioactive compounds in Rhus verniciflua extracts using high-performance liquid chromatography-tandem mass spectrometry. The chromatographic separation was performed using a C-18 column under gradient elution with 0.1% formic acid and acetonitrile as the mobile phase solvents. The analyteswere detected in the negative-ion mode using multiple-reaction monitoring detection with an electrospray ionization interface. The calibration curves for all the analytes showed good linearity (r(2) ˃ 0.997) over the concentration range of 1-1,000 ng/mL. The recovery valueswere within 89.53-110.14%, and the intra- and interday coefficients of variation were ˂4.86% for all the tested compounds. The developed method was successfully applied to a quality assessment of the R. verniciflua extract samples.
URI
https://academic.oup.com/chromsci/article/54/3/390/2754784http://hdl.handle.net/20.500.11754/50194
ISSN
0021-9665; 1945-239X
DOI
10.1093/chromsci/bmv152
Appears in Collections:
COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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