Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 박유신 | - |
dc.date.accessioned | 2018-03-21T07:24:11Z | - |
dc.date.available | 2018-03-21T07:24:11Z | - |
dc.date.issued | 2014-04 | - |
dc.identifier.citation | African Journal of Pharmacy and Pharmacology, Vol. 8, 13, pp. 356-363, | en_US |
dc.identifier.issn | 1996-0816 | - |
dc.identifier.uri | http://www.academicjournals.org/journal/AJPP/article-abstract/E35A32943942 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/50131 | - |
dc.description.abstract | A simple, rapid and selective liquid chromatography coupled with tandem mass spectrometry (LCMS/MS) is developed and validated for quantification of propranolol without the sample extraction step in rat plasma using tramadol as an internal standard (IS). The analytes are separated using an isocraticmobile phase which consist of methanol and 10 mm ammonium formate (70/30, v/v) on an isocratic UKC18 (Imtakt Unison 2.0 × 50 mm, 3 μm) column and was analyzed by MS/MS in the multiple reaction monitoring (MRM) mode using the transitions of respective (M+H)+ ions, m/z 260.0→116.2 and m/z264.2→58.2 for quantification of propranolol and IS, respectively. The standard calibration curves showed good linearity within the range of 2.0 to 800.0 ng/ml (r2 = 0.999, 1/x2 weight). The lower limit of quantification (LLOQ) was 2 ng/ml. The retention time of propranolol and IS were 1.12 and 0.939 minwhich means that it is the potential for the high-through put potential of the proposed method. In addition, no significant metabolic compounds were found to interfere with the analysis. Acceptable precision and accuracy were obtained for the concentrations over the standard curve range. Thevalidated method was successfully applied for the pharmacokinetic studies after 2 mg/kg of propranolol HCl in the thioacetamide (TAA)-induced fibrotic rats. | en_US |
dc.description.sponsorship | This work was supported by the MK Pharmaceutical Co.South Korea and Hanyang Biomedical Research Institute, Hanyang University, South Korea. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Academic Journals | en_US |
dc.subject | Propranolol | en_US |
dc.subject | liquid chromatography | en_US |
dc.subject | mass spectrometry | en_US |
dc.subject | pharmacokinetics | en_US |
dc.subject | thioacetamide | en_US |
dc.subject | liver fibrosis | en_US |
dc.title | Quantification of propranolol in rat plasma by LC-MS/MS using tramadol as an internal standard: Application to pharmacokinetic studies in TAA-induced liver fibrotic rats | en_US |
dc.title.alternative | MS using tramadol as an internal standard: Application to pharmacokinetic studies in TAA-induced liver fibrotic rats | en_US |
dc.type | Article | en_US |
dc.relation.no | 13 | - |
dc.relation.volume | 8 | - |
dc.identifier.doi | 10.5897/AJPP2013. 3780 | - |
dc.relation.page | 356-363 | - |
dc.relation.journal | African Journal of Pharmacy and Pharmacology | - |
dc.contributor.googleauthor | Kang, Hyun-Ku | - |
dc.contributor.googleauthor | Kang, Min-A | - |
dc.contributor.googleauthor | Kim, Hyun-Jin | - |
dc.contributor.googleauthor | Park, Yoo-Sin | - |
dc.contributor.googleauthor | Kim, Shin-Hee | - |
dc.contributor.googleauthor | Kim, Do-Wan | - |
dc.contributor.googleauthor | Kang, Ju-Seop | - |
dc.relation.code | 2014002814 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF HUMAN ECOLOGY[S] | - |
dc.sector.department | DEPARTMENT OF FOOD & NUTRITION | - |
dc.identifier.pid | hidocys | - |
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