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Deletion Variants of RABGAP1L, 10q21.3, and C4 Are Associated With the Risk of Systemic Lupus Erythematosus in Korean Women

Title
Deletion Variants of RABGAP1L, 10q21.3, and C4 Are Associated With the Risk of Systemic Lupus Erythematosus in Korean Women
Author
방소영
Keywords
article; chromosome 10q; chromosome 1q; chromosome 8q; disease predisposition; female; gene deletion; gene replication; genetic association; genetic risk; genotype; high risk patient; human; Korea; major clinical study; polymerase chain reaction; priority journal
Issue Date
2013-04
Publisher
American College of Rheumatology
Citation
ARTHRITIS AND RHEUMATISM, 2013, 65(4), p1055 ~ 1063
Abstract
Abstract Objective Several copy number variations (CNVs) have been found to be associated with systemic lupus erythematosus (SLE) through the target gene approach. However, genome?wide features of CNVs and their role in the risk of SLE remain unknown. The aim of this study was to identify SLE?associated CNVs in Korean women. Methods Genome?wide assessments of CNVs were performed in 382 SLE patients and 191 control subjects, using an Illumina HumanHap610 BeadChip genotyping platform. SLE?associated CNV regions that were identified by genome?wide association study (GWAS) were replicated in quantitative polymerase chain reaction (PCR) and deletion?typing PCR analyses in an independent sample set comprising 564 SLE patients and 511 control subjects. Results Of 144 common CNV regions, 3 deletion?type CNV regions in 1q25.1, 8q23.3, and 10q21.3 were found to be significantly associated with SLE by GWAS analysis. In the independent replication, the CNV regions in 1q25.1 (RABGAP1L) and 10q21.3 were successfully replicated (odds ratio [OR] 1.30, P = 0.038 and OR 1.90, P = 3.6 × 10?5, respectively), and the associations were confirmed again by deletion?typing PCR. The CNV region in the C4 gene, which showed a potential association in the discovery stage, was included in the replication analysis and was found to be significantly associated with the risk of SLE (OR 1.88, P = 0.01). Through deletion?typing PCR, the exact sizes and breakpoint sequences of the deletions were defined. Individuals with the deletions in all 3 loci (RABGAP1L, 10q21.3, and C4) had a much higher risk of SLE than did those without any deletions in the 3 loci (OR 5.52, P = 3.9 × 10?4). Conclusion These CNV regions can be useful to identify the pathogenic mechanisms of SLE, and might be used to more accurately predict the risk of SLE by taking into consideration their synergistic effects on disease susceptibility.
URI
http://onlinelibrary.wiley.com/doi/10.1002/art.37854/abstract?systemMessage=Wiley+Online+Library+is+migrating+to+a+new+platform+powered+by+Atypon%2C+the+leading+provider+of+scholarly+publishing+platforms.+The+new+Wiley+Online+Library+will+be+migrated+over+the+weekend+of+March+17+and+18.You+should+not+experience+any+issues+or+loss+of+access+during+this+time.+For+more+information%2C+please+visit+our+migration+page%3A++http%3A%2F%2Fwww.wileyactual.com%2FWOLMigration%2Fhttp://hdl.handle.net/20.500.11754/49972
ISSN
0004-3591
DOI
10.1002/art.37854
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > ETC
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