Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김철영 | - |
dc.date.accessioned | 2018-03-20T08:14:55Z | - |
dc.date.available | 2018-03-20T08:14:55Z | - |
dc.date.issued | 2016-02 | - |
dc.identifier.citation | JOURNAL OF APPLIED PHYCOLOGY, v. 28, No. 1, Page. 181-189 | en_US |
dc.identifier.issn | 0921-8971 | - |
dc.identifier.issn | 1573-5176 | - |
dc.identifier.uri | https://link.springer.com/article/10.1007/s10811-015-0548-y | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/49806 | - |
dc.description.abstract | A freshwater alga, Spirogyra sp., collected in shallow ponds in South Korea, was evaluated for its antilipid peroxidative effect against 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH)-induced in vivo zebrafish, and antioxidative compounds from the alga were efficiently identified using 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS(+)) online high-performance liquid chromatography (HPLC) and preparative centrifugal partition chromatography. The ethyl acetate fraction of Spirogyra sp. (SPE) in each fraction showed the strongest 2,2-diphenyl-1-picrylhydrazyl scavenging activity and significantly scavenged 2',7'-dichlorodihydrofluorescein diacetate and diphenyl-1-pyrenylphosphine fluorescence, respectively, in AAPH-induced zebrafish embryo without any cytotoxicity in the concentrations between 25 and 50 mu g mL(-1). The two main antioxidative compounds in SPE were confirmed by ABTS(+) online HPLC and were identified as gallic acid and methyl gallate, respectively, by HPLC-diode array detection (DAD)-electrospray ionization (ESI)/mass spectrometry (MS), H-1- and C-13-NMR. We conclude therefore that Spirogyra sp. is rich in gallic acid and methyl gallate, and it might be useful as a strong antilipid peroxidation material. | en_US |
dc.description.sponsorship | This research was supported by the Ministry of Agriculture, Food and Rural Affairs (MAFRA), Ministry of Oceans and Fisheries (MOF), Rural Development Administration (RDA), and Korea Forest Service (KFS) (213004-04-2-SB930) | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | SPRINGER | en_US |
dc.subject | Spirogyra sp. | en_US |
dc.subject | Antilipid peroxidation | en_US |
dc.subject | ABTS(+) online HPLC | en_US |
dc.subject | Centrifugal partition chromatography (CPC) | en_US |
dc.subject | Zebrafish | en_US |
dc.title | Protective effect of a freshwater alga, Spirogyra sp., against lipid peroxidation in vivo zebrafish and purification of antioxidative compounds using preparative centrifugal partition chromatography | en_US |
dc.type | Article | en_US |
dc.relation.no | 1 | - |
dc.relation.volume | 28 | - |
dc.identifier.doi | 10.1007/s10811-015-0548-y | - |
dc.relation.page | 181-189 | - |
dc.relation.journal | JOURNAL OF APPLIED PHYCOLOGY | - |
dc.contributor.googleauthor | Lee, Ji-Hyeok | - |
dc.contributor.googleauthor | Han, Jong Won | - |
dc.contributor.googleauthor | Ko, Ju-Young | - |
dc.contributor.googleauthor | Lee, Wonwoo | - |
dc.contributor.googleauthor | Ahn, Ginnae | - |
dc.contributor.googleauthor | Kim, Chul-Young | - |
dc.contributor.googleauthor | Kim, Gwang Hoon | - |
dc.contributor.googleauthor | Jeon, You-Jin | - |
dc.relation.code | 2016000267 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF PHARMACY[E] | - |
dc.sector.department | DEPARTMENT OF PHARMACY | - |
dc.identifier.pid | chulykim | - |
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