Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 한중수 | - |
dc.date.accessioned | 2018-03-20T07:31:03Z | - |
dc.date.available | 2018-03-20T07:31:03Z | - |
dc.date.issued | 2012-04 | - |
dc.identifier.citation | Molecular Neurobiology, 2012, 45(3), P.507-519 | en_US |
dc.identifier.issn | 0893-7648 | - |
dc.identifier.uri | https://link.springer.com/article/10.1007%2Fs12035-012-8268-7 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/49748 | - |
dc.description.abstract | The purpose of this study was to examine the role of phospholipase D1 (PLD1) in basic fibroblast growth factor (bFGF)-induced neurotrophin-3 (NT-3) expression and neurite outgrowth in H19-7 rat hippocampal neuronal progenitor cells. Overexpression of PLD1 increased bFGF-induced NT-3 expression, and dominant-negative-PLD1 or PLD1 siRNA abolished bFGF-induced NT-3 expression and neurite outgrowth. Treatment with bFGF activated the RhoA/Rho-associated kinase (ROCK)/c-jun N-terminal kinase (JNK) pathway, and bFGF-induced NT-3 expression was blocked by a dominant-negative RhoA as well as by a specific Rho-kinase inhibitor (Y27632) and a SAPK/JNK inhibitor (SP600125). Furthermore, bFGF-induced JNK activation was also blocked by Y27632. These results indicate that the RhoA/ROCK/JNK pathway acts as an upstream signaling pathway in bFGF-induced NT-3 expression. Also, phosphatidic acid, the product of PLD, increased NT-3 expression. We found that PLD regulated the RhoA/ROCK/JNK pathway, which then led to Elk-1 transactivation. When Elk-1 activity was blocked by Elk-1 siRNA, bFGF-induced NT-3 expression and neurite outgrowth decreased. NT-3 overexpression increased neurite outgrowth, indicating that NT-3 is important for neurite outgrowth. Taken together, these results suggest that PLD1 is an important regulator of bFGF-induced NT-3 expression and neurite outgrowth, which are mediated by the RhoA/ROCK/JNK pathway via Elk-1 in H19-7 cells. | en_US |
dc.description.sponsorship | This study was supported by the National Research Foundation of Korea (NRF) grant funded by the Ministry of Education, Science, and Technology of Korea government (MEST,2010-0029503; Mid-career Researcher Program, 2010-0026844). | en_US |
dc.language.iso | ko_KR | en_US |
dc.publisher | Humana Press Inc | en_US |
dc.subject | Phospholipase D1 | en_US |
dc.subject | RhoA | en_US |
dc.subject | JNK | en_US |
dc.subject | Elk-1 | en_US |
dc.subject | NT-3 | en_US |
dc.subject | Neurite outgrowth | en_US |
dc.title | Phospholipase D1 is an Important Regulator of bFGF-Induced Neurotrophin-3 Expression and Neurite Outgrowth in H19-7 Cells | en_US |
dc.type | Article | en_US |
dc.relation.no | 3 | - |
dc.relation.volume | 45 | - |
dc.identifier.doi | 10.1007/s12035-012-8268-7 | - |
dc.relation.page | 507-519 | - |
dc.relation.journal | MOLECULAR NEUROBIOLOGY | - |
dc.contributor.googleauthor | Choi, Hye-Jin | - |
dc.contributor.googleauthor | Chang, Brian Junho | - |
dc.contributor.googleauthor | Han, Joong-Soo | - |
dc.relation.code | 2012206827 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | jshan | - |
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