Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김형범 | - |
dc.date.accessioned | 2018-03-20T06:29:30Z | - |
dc.date.available | 2018-03-20T06:29:30Z | - |
dc.date.issued | 2014-05 | - |
dc.identifier.citation | NATURE REVIEWS GENETICS, 권: 15, 호: 5, 페이지: 321-334 | en_US |
dc.identifier.issn | 1471-0056 | - |
dc.identifier.issn | 1471-0064 | - |
dc.identifier.uri | http://www.nature.com/articles/nrg3686 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/49646 | - |
dc.description.abstract | Programmable nucleases - including zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and RNA-guided engineered nucleases (RGENs) derived from the bacterial clustered regularly interspaced short palindromic repeat (CRISPR)-Cas (CRISPR-associated) system - enable targeted genetic modifications in cultured cells, as well as in whole animals and plants. The value of these enzymes in research, medicine and biotechnology arises from their ability to induce site-specific DNA cleavage in the genome, the repair (through endogenous mechanisms) of which allows high-precision genome editing. However, these nucleases differ in several respects, including their composition, targetable sites, specificities and mutation signatures, among other characteristics. Knowledge of nuclease-specific features, as well as of their pros and cons, is essential for researchers to choose the most appropriate tool for a range of applications. | en_US |
dc.description.sponsorship | This work was supported by the National Research Foundation of Korea (2013-000718 to J.S.K. and 2011-0013568 to H. K.), Health Technology Research and Development Project by the Korean Ministry of Health and Welfare (H10C1740 to H. K.), and Converging Research Center Program funded by the Korean Ministry of Science, ICT and Future Planning (2013K000275 to H. K.). The authors thank three anonymous referees for comments and all the researchers, including our collaborators and competitors, who have contributed to the development of programmable nucleases in the past 20 years and regret the need to omit many relevant papers owing to the page limitation. | en_US |
dc.language.iso | en | en_US |
dc.publisher | NATURE PUBLISHING GROUP, MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND | en_US |
dc.subject | ZINC-FINGER NUCLEASES | en_US |
dc.title | A guide to genome engineering with programmable nucleases | en_US |
dc.type | Article | en_US |
dc.relation.volume | 15 | - |
dc.identifier.doi | 10.1038/nrg3686 | - |
dc.relation.page | 321-334 | - |
dc.relation.journal | NATURE REVIEWS GENETICS | - |
dc.contributor.googleauthor | Kim, Hyongbum | - |
dc.contributor.googleauthor | Kim, Jin-Soo | - |
dc.relation.code | 2014036472 | - |
dc.sector.campus | S | - |
dc.sector.daehak | GRADUATE SCHOOL OF BIOMEDICAL SCIENCE AND ENGINEERING[S] | - |
dc.identifier.pid | hkim1 | - |
dc.identifier.orcid | http://orcid.org/0000-0002-4693-738X | - |
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