Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이민형 | - |
dc.date.accessioned | 2018-03-20T05:41:21Z | - |
dc.date.available | 2018-03-20T05:41:21Z | - |
dc.date.issued | 2014-02 | - |
dc.identifier.citation | JOURNAL OF DRUG TARGETING; FEB 2014, 22, 2, p156-p164 | en_US |
dc.identifier.issn | 1061-186X | - |
dc.identifier.uri | https://www.tandfonline.com/doi/abs/10.3109/1061186X.2013.850502 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/49558 | - |
dc.description.abstract | Combined delivery of chemical drug and therapeutic gene has been introduced as an efficient method for the treatment of cancers such as glioblastoma. In this study, bis-chloroethylnitrosourea (BCNU) and vascular endothelial growth factor (VEGF) small interfering RNA (VEGFsiRNA) were co-delivered into C6 glioblastoma cells using a non-toxic peptide-based carrier. The R3V6 peptides, which are composed of 3-arginine and 6-valine, formed self-assembled micelles in aqueous solution. BCNU, a hydrophobic anti-cancer drug, was loaded into the hydrophobic core of the micelles, forming BCNU-loaded R3V6 micelles (R3V6-BCNU). In gel retardation assay, R3V6-BCNU formed a stable complex with siRNA. In vitro transfection assay showed that the VEGF-siRNA/R3V6-BCNU complex had the highest transfection efficiency into C6 cells at a 1: 20 weight ratio (VEGF-siRNA: R3V6-BCNU). In addition, the VEGF-siRNA/R3V6BCNU complexes had higher delivery efficiency than lipofectamine or naked siRNA. VEGF expressions were remarkably decreased by transfection of the VEGF-siRNA/R3V6 or VEGFsiRNA/ R3V6-BCNU complexes. Furthermore, R3V6-BCNU delivered BCNU more efficiently into the cells than BCNU only. Therefore, R3V6 delivered both VEGF-siRNA and BCNU efficiently into the glioblastoma cells. The results suggest that R3V6-BCNU may be useful for combined delivery of siRNA and chemical drug into cancer cells. | en_US |
dc.language.iso | en | en_US |
dc.publisher | INFORMA HEALTHCARE | en_US |
dc.subject | Combined delivery | en_US |
dc.subject | glioblastoma | en_US |
dc.subject | peptide micelles | en_US |
dc.subject | VEGF siRNA | en_US |
dc.title | Combined delivery of BCNU and VEGF siRNA using amphiphilic peptides for glioblastoma | en_US |
dc.type | Article | en_US |
dc.relation.no | 2 | - |
dc.relation.volume | 22 | - |
dc.identifier.doi | 10.3109/1061186X.2013.850502 | - |
dc.relation.page | 156-164 | - |
dc.relation.journal | JOURNAL OF DRUG TARGETING | - |
dc.contributor.googleauthor | Yi, Na | - |
dc.contributor.googleauthor | Oh, Binna | - |
dc.contributor.googleauthor | Kim, Hyun Ah | - |
dc.contributor.googleauthor | Lee, Minhyung | - |
dc.relation.code | 2014032966 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | minhyung | - |
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