Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이상훈 | - |
dc.date.accessioned | 2018-03-20T01:30:59Z | - |
dc.date.available | 2018-03-20T01:30:59Z | - |
dc.date.issued | 2014-02 | - |
dc.identifier.citation | Human Molecular Genetics, 2014, Vol. 23, No. 3, 657?667 | en_US |
dc.identifier.issn | 1460-2083 | - |
dc.identifier.issn | 0964-6906 | - |
dc.identifier.uri | https://academic.oup.com/hmg/article/23/3/657/592781 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/49263 | - |
dc.description.abstract | DNA methylation and hydroxymethylation have been implicated in normal development and differentiation, but our knowledge is limited about the genome-wide distribution of 5-methylcytosine (5 mC) and 5-hydroxymethylcytosine (5 hmC) during cellular differentiation. Using an in vitro model system of gradual differentiation of human embryonic stem (hES) cells into ventral midbrain-type neural precursor cells and terminally into dopamine neurons, we observed dramatic genome-wide changes in 5 mC and 5 hmC patterns during lineage commitment. The 5 hmC pattern was dynamic in promoters, exons and enhancers. DNA hydroxymethylation within the gene body was associated with gene activation. The neurogenesis-related genes NOTCH1, RGMA and AKT1 acquired 5 hmC in the gene body and were up-regulated during differentiation. DNA methylation in the promoter was associated with gene repression. The pluripotency-related genes POU5F1, ZFP42 and HMGA1 acquired 5 mC in their promoters and were down-regulated during differentiation. Promoter methylation also acted as a locking mechanism to maintain gene silencing. The mesoderm development-related genes NKX2-8, TNFSF11 and NFATC1 acquired promoter methylation during neural differentiation even though they were already silenced in hES cells. Our findings will help elucidate the molecular mechanisms underlying lineage-specific differentiation of pluripotent stem cells during human embryonic development. | en_US |
dc.description.sponsorship | This work was supported by grants from the Stem Cell Research Program (2012M3A9B4027954), the Future-Based Technology Development Program (NRF2011-0015710) and the KRIBB Research Initiative Program, which are funded by the Korean Ministry of Education, Science and Technology. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Oxford University Press | en_US |
dc.title | Dynamic changes in DNA methylation and hydroxymethylation when hES cells undergo differentiation toward a neuronal lineage | en_US |
dc.type | Article | en_US |
dc.relation.no | 3 | - |
dc.relation.volume | 23 | - |
dc.identifier.doi | 10.1093/hmg/ddt453 | - |
dc.relation.page | 657-667 | - |
dc.relation.journal | HUMAN MOLECULAR GENETICS | - |
dc.contributor.googleauthor | Kim, Mirang | - |
dc.contributor.googleauthor | Park, Young-Kyu | - |
dc.contributor.googleauthor | Kang, Tae-Wook | - |
dc.contributor.googleauthor | Lee, Sang-Hun | - |
dc.contributor.googleauthor | Rhee, Yong-Hee | - |
dc.contributor.googleauthor | Park, Jong-Lyul | - |
dc.contributor.googleauthor | Kim, Hee-Jin | - |
dc.contributor.googleauthor | Lee, Daeyoup | - |
dc.contributor.googleauthor | Lee, Doheon | - |
dc.contributor.googleauthor | Kim, Seon-Young | - |
dc.relation.code | 2014030592 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | leesh | - |
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