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dc.contributor.author이영식-
dc.date.accessioned2018-03-19T04:41:19Z-
dc.date.available2018-03-19T04:41:19Z-
dc.date.issued2016-01-
dc.identifier.citationGENE, v. 576, No. 1, Page. 119-125en_US
dc.identifier.issn0378-1119-
dc.identifier.issn1879-0038-
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0378111915011981-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/48839-
dc.description.abstractFetal alcohol spectrum disorder is a collective term that represents fetal abnormalities associated with maternal alcohol consumption. Prenatal alcohol exposure and related anomalies are well characterized, but the molecular mechanism behind this phenomenon is not yet understood. Few insights have been gained from genetic and epigenetic studies of fetal alcohol spectrum disorder. Our aim was to profile the important molecular regulators of ethanol-related alterations of the genome. For this purpose, we have analyzed the gene expression pattern of human carcinoma cell-derived embryoid bodies in the absence or presence of ethanol. A cDNA microarray analysis was used to profile mRNA expression in embryoid bodies at day 7 with or without ethanol treatment. A total of 493 differentially expressed genes were identified in response to 50 mM ethanol exposure. Of these, 111 genes were up-regulated, and 382 were down-regulated. Gene ontology term enrichment analysis revealed that these genes are involved in important biological processes: neurological system processes, cognition, behavior, sensory perception of smell, taste and chemical stimuli and synaptic transmission. Similarly, the enrichment of disease related genes included relevant categories such as neurological diseases, developmental disorders, skeletal and muscular disorders, and connective tissue disorders. Furthermore, we have identified a group of 26 genes that encode transcription factors. We validated the relative gene expression of several transcription factors using quantitative real time PCR. We hope that our study substantially contributes to the understanding of the molecular mechanisms underlying the pathology of alcohol-mediated anomalies and facilitates further research. (C) 2015 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipThis work was supported by part of the Korean Alcohol Research Foundation, and by part of the National Research Foundation of Korea (NRF) grant funded by the Korea Government (MSIP) (2013R1A1A3011026 to K.H.J, and 2011-0030049 to Y.G.C.).en_US
dc.language.isoen_USen_US
dc.publisherELSEVIER SCIENCE BVen_US
dc.subjectAlcohol consumptionen_US
dc.subjectFASDen_US
dc.subjectMicroarray analysisen_US
dc.subjectGene expressionen_US
dc.subjectTranscription factorsen_US
dc.subjectFETAL-ALCOHOL-SYNDROMEen_US
dc.subjectSTEM-CELLSen_US
dc.subjectMICROARRAY ANALYSISen_US
dc.subjectGENE-EXPRESSIONen_US
dc.subjectDIFFERENTIATIONen_US
dc.subjectDIAGNOSISen_US
dc.subjectNETWORKSen_US
dc.subjectINSIGHTSen_US
dc.subjectMICEen_US
dc.titleProfiling ethanol-targeted transcription factors in human carcinoma cell-derived embryoid bodiesen_US
dc.typeArticleen_US
dc.relation.no1-
dc.relation.volume576-
dc.identifier.doi10.1016/j.gene.2015.09.085-
dc.relation.page119-125-
dc.relation.journalGENE-
dc.contributor.googleauthorMandal, Chanchal-
dc.contributor.googleauthorHalder, Debasish-
dc.contributor.googleauthorChai, Jin Choul-
dc.contributor.googleauthorLee, Young Seek-
dc.contributor.googleauthorJung, Kyoung Hwa-
dc.contributor.googleauthorChai, Young Gyu-
dc.relation.code2016001133-
dc.sector.campusE-
dc.sector.daehakCOLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E]-
dc.sector.departmentDEPARTMENT OF MOLECULAR AND LIFE SCIENCE-
dc.identifier.pidyslee-
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COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > ETC
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