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dc.contributor.author김동욱-
dc.date.accessioned2018-03-19T04:37:26Z-
dc.date.available2018-03-19T04:37:26Z-
dc.date.issued2016-01-
dc.identifier.citationFRONTIERS IN MICROBIOLOGY, v. 6, Page. 1548-1548en_US
dc.identifier.issn1664-302X-
dc.identifier.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4709847-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/48834-
dc.description.abstractWe have developed a novel Neisseria meningitidis serogroup-specific loop-mediated isothermal amplification (LAMP) assay for six of the most common meningococcal serogroups (A, B, C, W, X, and Y). The assay was evaluated using a set of 31 meningococcal LAMP assay positive cerebrospinal fluid (CSF) specimens from 1574 children with suspected meningitis identified in prospective surveillance between 1998 and 2002 in Vietnam, China, and Korea. Primer specificity was validated using 15 N. meningitidis strains (including serogroups A, B, C, E, W, X, Y, and Z) and 19 non-N. meningitidis species. The N. meningitidis serogroup LAMP detected down to ten copies and 100 colony-forming units per reaction. Twenty-nine CSF had N. meningitidis serogroup identified by LAMP compared with two CSF in which N. meningitidis serogroup was identified by culture and multi-locus sequence typing. This is the first report of a serogroup-specific identification assay for N. meningitidis using the LAMP method. Our results suggest that this assay will be a rapid, sensitive, and uniquely serogroup-specific assay with potential for application in clinical laboratories and public health surveillance systems.en_US
dc.language.isoen_USen_US
dc.publisherFRONTIERS MEDIA SAen_US
dc.subjectNeisseria meningitidisen_US
dc.subjectcerebrospinal fluiden_US
dc.subjectchildrenen_US
dc.subjectloop-mediated isothermal amplificationen_US
dc.subjectmeningitisen_US
dc.subjectserogroup identificationen_US
dc.titleA novel loop-mediated isothermal amplification assay for serogroup identification of Neisseria meningitidis in cerebrospinal fluiden_US
dc.typeArticleen_US
dc.relation.volume6-
dc.identifier.doi10.3389/fmicb.2015.01548-
dc.relation.page1548-1548-
dc.relation.journalFRONTIERS IN MICROBIOLOGY-
dc.contributor.googleauthorLee, D-
dc.contributor.googleauthorKim, EJ-
dc.contributor.googleauthorKilgore, PE-
dc.contributor.googleauthorTakahashi, H-
dc.contributor.googleauthorOhnishi, M-
dc.contributor.googleauthorTomono, J-
dc.contributor.googleauthorMiyamoto, S-
dc.contributor.googleauthorOmagari, D-
dc.contributor.googleauthorKim, DW-
dc.contributor.googleauthorSeki, M-
dc.relation.code2016012485-
dc.sector.campusE-
dc.sector.daehakCOLLEGE OF PHARMACY[E]-
dc.sector.departmentDEPARTMENT OF PHARMACY-
dc.identifier.piddongwook-
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COLLEGE OF PHARMACY[E](약학대학) > ETC
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