뉴트라비딘 검출용 간이 진단키트의 성능향상을 위한 기본 플랫폼 선정
- Title
- 뉴트라비딘 검출용 간이 진단키트의 성능향상을 위한 기본 플랫폼 선정
- Other Titles
- The selection of basic platform for improving the sensitivity of neutravidin rapid detection kit
- Author
- 엄애선
- Keywords
- Rapid detection kit; Nitrocellulose membrane; Avidin-biotin interaction
- Issue Date
- 2012-12
- Publisher
- 충남대학교 농업과학연구소
- Citation
- 농업과학연구, 2012, 39(4), P.613 - 618, 6P.
- Abstract
- This study was performed to optimize the basic platform of a lateral flow immunoassay. Improvement of the limit of detection (LOD) was evaluated according to the width of a nitrocellulose membrane with varying concentrations of analyte. The analyte, neutravidin was detected based on the avidin-biotin interaction. The antibody-Au nanoparticle conjugation was mostly stabled in a PBS buffer of pH 7.3. The optimal widths of a nitrocellulose membrane were 4 and 6 mm considering the sample flow rate and signal strength of the test line on the membrane. The LOD of neutravidin was 0.001 mg/ml in the optimum conditions.
- URI
- http://www.dbpia.co.kr/Journal/ArticleDetail/NODE02065131http://hdl.handle.net/20.500.11754/48675
- ISSN
- 1225-2220; 2466-2402
- Appears in Collections:
- COLLEGE OF HUMAN ECOLOGY[S](생활과학대학) > ETC
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