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DC FieldValueLanguage
dc.contributor.author황경균-
dc.date.accessioned2018-03-16T08:19:04Z-
dc.date.available2018-03-16T08:19:04Z-
dc.date.issued2012-08-
dc.identifier.citationRestorative Dentistry & Endodontics, 2012, 37(3), P.142-148, 7P.en_US
dc.identifier.issn1225-0864-
dc.identifier.issn2234-7658-
dc.identifier.issn2234-7666-
dc.identifier.urihttps://synapse.koreamed.org/DOIx.php?id=10.5395/rde.2012.37.3.142-
dc.description.abstractObjectives: We analyzed gene-expression profiles after 14 day odontogenic induction of human dental pulp cells (DPCs) using a DNA microarray and sought candidate genes possibly associated with mineralization. Materials and Methods: Induced human dental pulp cells were obtained by culturing DPCs in odontogenic induction medium (OM) for 14 day. Cells exposed to normal culture medium were used as controls. Total RNA was extracted from cells and analyzed by microarray analysis and the key results were confirmed selectively by reverse-transcriptase polymerase chain reaction (RT-PCR). We also performed a gene set enrichment analysis (GSEA) of the microarray data. Results: Six hundred and five genes among the 47,320 probes on the BeadChip differed by a factor of more than two-fold in the induced cells. Of these, 217 genes were upregulated, and 388 were down-regulated. GSEA revealed that in the induced cells, genes implicated in Apoptosis and Signaling by wingless MMTV integration (Wnt) were significantly upregulated. Conclusions: Genes implicated in Apoptosis and Signaling by Wnt are highly connected to the differentiation of dental pulp cells into odontoblast.en_US
dc.description.sponsorshipThis study was supported by Biostart Program from graduate school of biomedical science and engineering, Hanyang University.en_US
dc.language.isoenen_US
dc.publisher大韓齒科保存學會(Korean Academy of Conservative Dentistry)en_US
dc.subjectDifferentiationen_US
dc.subjectGene set enrichment analysisen_US
dc.subjectHuman dental pulp cellen_US
dc.subjectMicroarrayen_US
dc.titleAnalysis of gene expression during odontogenic differentiation of cultured human dental pulp cellsen_US
dc.typeArticleen_US
dc.relation.no3-
dc.relation.volume37-
dc.identifier.doi10.5395/rde.2012.37.3.142-
dc.relation.page142-148-
dc.relation.journalRestorative Dentistry and Endodontics-
dc.contributor.googleauthorSeo, Min-Seock-
dc.contributor.googleauthorHwang, Kyung-Gyun-
dc.contributor.googleauthorKim, Hyong-Bum-
dc.contributor.googleauthorBaek, Seung-Ho-
dc.contributor.googleauthor서민석-
dc.contributor.googleauthor황경균-
dc.contributor.googleauthor김형범-
dc.contributor.googleauthor백승호-
dc.relation.code2012285644-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF MEDICINE[S]-
dc.sector.departmentDEPARTMENT OF MEDICINE-
dc.identifier.pidhkg-


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