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Analysis of gene expression during odontogenic differentiation of cultured human dental pulp cells

Title
Analysis of gene expression during odontogenic differentiation of cultured human dental pulp cells
Author
황경균
Keywords
Differentiation; Gene set enrichment analysis; Human dental pulp cell; Microarray
Issue Date
2012-08
Publisher
大韓齒科保存學會(Korean Academy of Conservative Dentistry)
Citation
Restorative Dentistry & Endodontics, 2012, 37(3), P.142-148, 7P.
Abstract
Objectives: We analyzed gene-expression profiles after 14 day odontogenic induction of human dental pulp cells (DPCs) using a DNA microarray and sought candidate genes possibly associated with mineralization. Materials and Methods: Induced human dental pulp cells were obtained by culturing DPCs in odontogenic induction medium (OM) for 14 day. Cells exposed to normal culture medium were used as controls. Total RNA was extracted from cells and analyzed by microarray analysis and the key results were confirmed selectively by reverse-transcriptase polymerase chain reaction (RT-PCR). We also performed a gene set enrichment analysis (GSEA) of the microarray data. Results: Six hundred and five genes among the 47,320 probes on the BeadChip differed by a factor of more than two-fold in the induced cells. Of these, 217 genes were upregulated, and 388 were down-regulated. GSEA revealed that in the induced cells, genes implicated in Apoptosis and Signaling by wingless MMTV integration (Wnt) were significantly upregulated. Conclusions: Genes implicated in Apoptosis and Signaling by Wnt are highly connected to the differentiation of dental pulp cells into odontoblast.
URI
https://synapse.koreamed.org/DOIx.php?id=10.5395/rde.2012.37.3.142
ISSN
1225-0864; 2234-7658; 2234-7666
DOI
10.5395/rde.2012.37.3.142
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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