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Failure of Tooth Formation Mediated by miR-135a Overexpression via BMP Signaling

Title
Failure of Tooth Formation Mediated by miR-135a Overexpression via BMP Signaling
Author
김계성
Keywords
microRNA; Bmp receptors; miRNA expression; lentivirus; miRNA inhibitor; tooth development
Issue Date
2014-06
Publisher
SAGE PUBLICATIONS INC
Citation
JOURNAL OF DENTAL RESEARCH, 2014, 93(6), P.571-575
Abstract
MicroRNAs (miRNAs) are known to regulate a variety of gene functions in many tissues and organs, but their expression and function in tooth development are not well-understood. A specific miRNA, miR-135a, was determined to be highly expressed at the bud stage. Interestingly, after the cap stage, miR-135a was expressed in the epithelium and mesenchyme but not in the inner enamel epithelium. To identify the relationship between miR-135a and its putative target genes, Bmpr-Ia and Bmpr-Ib, in early tooth development, miR-135a was ectopically overexpressed with a lentivirus. This overexpression resulted in the repression of Bmpr-Ia and Bmpr-Ib. Furthermore, miR-135a inhibited both Bmpr-Ia and Bmpr-Ib transcription. BMP2 proteins were expressed ectopically in tooth germs during the cap stage to determine the relationship between miR-135a and BMP signaling in early tooth development. When miR-135a was ectopically expressed, no tooth formation was observed after 4 wk of incubation in the kidney capsule. This study suggested that Bmp signaling, specifically Bmpr-Ia and Bmpr-Ib, regulates tooth formation via miR-135a.
URI
http://journals.sagepub.com/doi/abs/10.1177/0022034514529303?journalCode=jdrb
ISSN
0022-0345; 1544-0591
DOI
10.1177/0022034514529303
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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