Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 윤지희 | - |
dc.date.accessioned | 2018-03-16T00:03:54Z | - |
dc.date.available | 2018-03-16T00:03:54Z | - |
dc.date.issued | 2014-02 | - |
dc.identifier.citation | Immune Network, 2014, 14(1), P.38-44 | en_US |
dc.identifier.issn | 1598-2629 | - |
dc.identifier.issn | 2092-6685 | - |
dc.identifier.uri | https://synapse.koreamed.org/DOIx.php?id=10.4110/in.2014.14.1.38 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/47532 | - |
dc.description.abstract | K/BxN serum can transfer arthritis to normal mice owing to the abundant autoantibodies it contains, which trigger innate inflammatory cascades in joints. Little is known about whether gut-residing microbes affect host susceptibility to autoantibody-mediated arthritis. To address this, we fed C57BL/6 mice with water containing a mixture of antibiotics (ampicillin, vancomycin, neomycin, and metronidazol) for 2 weeks and then injected them with K/BxN serum. Antibiotic treatment significantly reduced the amount of bacterial genomic DNA isolated from fecal samples, in particular a gene encoding 16S ribosomal RNA derived from segmented filamentous bacteria. Arthritic signs, as indicated by the arthritic index and ankle thickness, were significantly attenuated in antibiotic-treated mice compared with untreated controls. Peyer's patches and mesenteric lymph nodes from antibiotic-treated mice contained fewer IL-17-expressing cells than those from untreated mice. Antibiotic treatment reduced serum C3 deposition in vitro via the alternative complement pathway. IL-17-/- congenic C57BL/6 mice were less susceptible to K/BxN serum-transferred arthritis than their wild-type littermates, but were still responsive to treatment with antibiotics. These results suggest that gut-residing microbes, including segmented filamentous bacteria, induce IL-17 production in GALT and complement activation via the alternative complement pathway, which cause the host to be more susceptible to autoantibody-mediated arthritis. | en_US |
dc.description.sponsorship | We thank Drs D. Mathis and Y. Iwakura for providing KRN mice and IL-17-/- mice, respectively. This work was supported by a National Research Foundation grant funded by the Korean Government (MEST; 2009-0081790) | en_US |
dc.language.iso | en | en_US |
dc.publisher | The Korean Association of Immunologists | en_US |
dc.subject | K/BxN serum-transferred arthritis | en_US |
dc.subject | Gut-residing microbes | en_US |
dc.subject | Antibiotics | en_US |
dc.subject | IL-17 | en_US |
dc.subject | Segmented filamentous bacteria | en_US |
dc.title | Gut-residing Microbes Alter the Host Susceptibility to Autoantibody-mediated Arthritis | en_US |
dc.type | Article | en_US |
dc.relation.no | 1 | - |
dc.relation.volume | 14 | - |
dc.identifier.doi | 10.4110/in.2014.14.1.38 | - |
dc.relation.page | 38-44 | - |
dc.relation.journal | Immune Network | - |
dc.contributor.googleauthor | Lee, Hyerim | - |
dc.contributor.googleauthor | Jin, Bo-Eun | - |
dc.contributor.googleauthor | Jang, Eunkyeong | - |
dc.contributor.googleauthor | Lee, Reum | - |
dc.contributor.googleauthor | Han, Dong Soo | - |
dc.contributor.googleauthor | Kim, Ho-Youn | - |
dc.contributor.googleauthor | Youn, Jeehee | - |
dc.relation.code | 2014001295 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | jhyoun | - |
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