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dc.contributor.authorRamakrishna Suresh-
dc.date.accessioned2018-03-15T12:21:12Z-
dc.date.available2018-03-15T12:21:12Z-
dc.date.issued2014-01-
dc.identifier.citationBiochemical and Biophysical Research Communications, Vol.443, No. 4, 24 January 2014, Pages 1206-1210en_US
dc.identifier.issn0006-291X-
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0006291X1302192X-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/47464-
dc.description.abstractThe transcription factor, Kruppel-like factor 4 (Klf4) plays a crucial role in generating induced pluripotent stem cells (iPSCs). As the ubiquitination and degradation of the Klf4 protein have been suggested to play an important role in its function, the identification of specific lysine sites that are responsible for protein degradation is of prime interest to improve protein stability and function. However, the molecular mechanism regulating proteasomal degradation of the Klf4 is poorly understood. In this study, both the analysis of Klf4 ubiquitination sites using several Klf4 deletion fragments and bioinformatics predictions showed that the lysine sites which are signaling for Klf4 protein degradation lie in its N-terminal domain (aa 1-296). The results also showed that Lys32, 52,232, and 252 of Klf4 are responsible for the proteolysis of the Klf4 protein. These results suggest that Klf4 undergoes proteasomal degradation and that these lysine residues are critical for Klf4 ubiquitination. (C) 2013 Elsevier Inc. All rights reserved.en_US
dc.description.sponsorshipThis work was supported by the National Research Foundation of Korea (NRF) Grant funded by the Korea government (MEST) (NRF-2011-0015312).en_US
dc.language.isoenen_US
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCE, 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USAen_US
dc.subjectOncogeneen_US
dc.subjectProteasomal degradationen_US
dc.subjectTumor suppressoren_US
dc.subjectUbiquitinen_US
dc.subjectUbiquitinationen_US
dc.subjectPLURIPOTENT STEM-CELLSen_US
dc.subjectKRUPPEL-LIKE FACTOR-4en_US
dc.subjectPROSTATE-CANCERen_US
dc.subjectTRANSCRIPTION FACTORen_US
dc.subjectTUMOR-SUPPRESSORen_US
dc.subjectUBIQUITINen_US
dc.subjectPROTEASOMEen_US
dc.subjectSIGNALSen_US
dc.subjectOCT4en_US
dc.subjectMYCen_US
dc.titleCritical lysine residues of Klf4 required for protein stabilization and degradationen_US
dc.typeArticleen_US
dc.relation.volume443-
dc.identifier.doi10.1016/j.bbrc.2013.12.121-
dc.relation.page1206-1210-
dc.relation.journalBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS-
dc.contributor.googleauthorLim, Key-Hwan-
dc.contributor.googleauthorKim, So-Ra-
dc.contributor.googleauthorRamakrishna, Suresh-
dc.contributor.googleauthorBaek, Kwang-Hyun-
dc.relation.code2014026012-
dc.sector.campusS-
dc.sector.daehakGRADUATE SCHOOL OF BIOMEDICAL SCIENCE AND ENGINEERING[S]-
dc.identifier.pidsuri28-
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