Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 한명수 | - |
dc.date.accessioned | 2018-03-15T06:58:40Z | - |
dc.date.available | 2018-03-15T06:58:40Z | - |
dc.date.issued | 2014-07 | - |
dc.identifier.citation | Harmful Algae, 2014, 37, P.133-141 | en_US |
dc.identifier.issn | 1568-9883 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S1568988314000754?via%3Dihub | - |
dc.description.abstract | While the toxic dinoflagellate Cochlodinium polykrikoides is known to form blooms that are maintained for extended periods, the genetic differentiation of these blooms are currently unknown. To assess this, we developed a real-time PCR assay to quantify C polykrikoides at the intra-specific level, and applied this assay to field samples collected in Korean coastal waters from summer through fall. Assays were successfully developed to target the large-subunit ribosomal RNA region of the three major ribotypes of C polykrikoides: Philippines, East Asian, and American/Malaysian. Significant linear relationships (r(2) >= 0.995) were established between C-t and the log of the copy number for each ribotype qPCR assay. Using these assays, C. polykrikoides blooms in Korean coastal waters were found to be comprised of Philippines and East Asian ribotypes but not the American/Malaysian ribotype. The Philippines ribotype was found to be highly abundant during summer bloom initiation and peak, whereas the East Asian ribotype became the dominant ribotype in the fall. As such, this newly developed qPCR assay can be used to quantify the cryptic ecological succession of sub-populations of C polykrikoides during blooms that light microscopy and previously developed qPCR assays cannot resolve. | en_US |
dc.description.sponsorship | We thank Dr. Ying Zhong Tang for providing the Cochlodinium polykrikoides strain CP01, Myo-Kyung Kim, Zhun Li for assistance with field work, and Dr. Jang-Seu Ki and Dr. Sunju Kim for providing kind comments. This research was supported by a grant from Marine Biotechnology Program Funded by Ministry of Oceans and Fisheries, Korea.[SS] | en_US |
dc.language.iso | en | en_US |
dc.publisher | Elsevier Science B.V | en_US |
dc.subject | Cochlodinium polykrikoides | en_US |
dc.subject | Ribotype | en_US |
dc.subject | Quantitative real-time PCR | en_US |
dc.subject | Field application | en_US |
dc.title | Resolving the intra-specific succession within Cochlodinium polykrikoides populations in southern Korean coastal waters via use of quantitative PCR assays | en_US |
dc.type | Article | en_US |
dc.relation.no | 0 | - |
dc.relation.volume | 37 | - |
dc.identifier.doi | 10.1016/j.hal.2014.04.019 | - |
dc.relation.page | 133-141 | - |
dc.relation.journal | HARMFUL ALGAE | - |
dc.contributor.googleauthor | Park, Bum Soo | - |
dc.contributor.googleauthor | Wang, Pengbin | - |
dc.contributor.googleauthor | Kim, Jin Ho | - |
dc.contributor.googleauthor | Kim, Joo-Hwan | - |
dc.contributor.googleauthor | Gobler, Christopher J | - |
dc.contributor.googleauthor | Han, Myung-Soo | - |
dc.relation.code | 2014030251 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF LIFE SCIENCE | - |
dc.identifier.pid | hanms | - |
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