Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ramakrishna Suresh | - |
dc.date.accessioned | 2018-03-15T04:44:22Z | - |
dc.date.available | 2018-03-15T04:44:22Z | - |
dc.date.issued | 2014-08 | - |
dc.identifier.citation | Methods, 2014, 69(1), p108-117 | en_US |
dc.identifier.issn | 1046-2023 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S1046202314001650?via%3Dihub | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/47063 | - |
dc.description.abstract | Targeted gene knockout using engineered nucleases such as transcription activator like-effector nucleases (TALENs) is a gold standard for investigating the functions of a gene of interest. Although most TALENs can cleave chromosomal DNA efficiently, the activities of designed TALENs are not always high enough to allow the efficient derivation of cells containing TALEN-driven mutations. Thus, simple methods to enrich cells containing TALEN-directed mutations would facilitate the use of TALENs. Here we describe the enrichment of such cells using surrogate episomal reporters coupled with flow cytometric sorting, magnetic separation, or hygromycin selection. | en_US |
dc.description.sponsorship | H.K. is supported in part by the National Research Foundation of Korea ( 2011-0013568 , 2011-0019357 , 2013M3A9B4076544 , 2008-0062287 ) and the Health Technology R&D Project, Ministry of Health and Welfare of Korea ( HI10C1740 ). | en_US |
dc.language.iso | en | en_US |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE, 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA | en_US |
dc.subject | Transcription activator-like effector nucleases | en_US |
dc.subject | Enrichment | en_US |
dc.subject | Mutant cells | en_US |
dc.subject | Flow cytometry | en_US |
dc.subject | Magnetic separation | en_US |
dc.subject | Hygromycin B | en_US |
dc.title | Enrichment of cells with TALEN-induced mutations using surrogate reporters | en_US |
dc.type | Article | en_US |
dc.relation.no | 1 | - |
dc.relation.volume | 69 | - |
dc.identifier.doi | 10.1016/j.ymeth.2014.04.012 | - |
dc.relation.page | 108-117 | - |
dc.relation.journal | METHODS | - |
dc.contributor.googleauthor | Kim, Young-Hoon | - |
dc.contributor.googleauthor | Ramakrishna, Suresh | - |
dc.contributor.googleauthor | Kim, Hyong-Bum | - |
dc.contributor.googleauthor | Kim, Jin-Soo | - |
dc.relation.code | 2014035976 | - |
dc.sector.campus | S | - |
dc.sector.daehak | GRADUATE SCHOOL OF BIOMEDICAL SCIENCE AND ENGINEERING[S] | - |
dc.identifier.pid | suri28 | - |
dc.identifier.researcherID | 24280276300 | - |
dc.identifier.orcid | 0000-0002-4693-738X | - |
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