Enrichment of cells with TALEN-induced mutations using surrogate reporters
- Title
- Enrichment of cells with TALEN-induced mutations using surrogate reporters
- Author
- Ramakrishna Suresh
- Keywords
- Transcription activator-like effector nucleases; Enrichment; Mutant cells; Flow cytometry; Magnetic separation; Hygromycin B
- Issue Date
- 2014-08
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE, 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA
- Citation
- Methods, 2014, 69(1), p108-117
- Abstract
- Targeted gene knockout using engineered nucleases such as transcription activator like-effector nucleases (TALENs) is a gold standard for investigating the functions of a gene of interest. Although most TALENs can cleave chromosomal DNA efficiently, the activities of designed TALENs are not always high enough to allow the efficient derivation of cells containing TALEN-driven mutations. Thus, simple methods to enrich cells containing TALEN-directed mutations would facilitate the use of TALENs. Here we describe the enrichment of such cells using surrogate episomal reporters coupled with flow cytometric sorting, magnetic separation, or hygromycin selection.
- URI
- https://www.sciencedirect.com/science/article/pii/S1046202314001650?via%3Dihubhttp://hdl.handle.net/20.500.11754/47063
- ISSN
- 1046-2023
- DOI
- 10.1016/j.ymeth.2014.04.012
- Appears in Collections:
- GRADUATE SCHOOL OF BIOMEDICAL SCIENCE AND ENGINEERING[S](의생명공학전문대학원) > ETC
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