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Isolation of extracellular vesicle from blood plasma using electrophoretic migration through porous membrane

Title
Isolation of extracellular vesicle from blood plasma using electrophoretic migration through porous membrane
Author
곽노균
Keywords
Exosome; Purification; Separation; Nanoporous membrane; Body fluid
Issue Date
2016-04
Publisher
ELSEVIER SCIENCE SA
Citation
SENSORS AND ACTUATORS B-CHEMICAL, v.223, page.289-297
Abstract
We present a high-yield electro-migration method to isolate extracellular vesicles (EVs), which achieves ultracentrifugation (U/C)-level size exclusion from biological fluids (e.g.,plasma).An electric field applied across a dialysis membrane with an appropriate pore size (30nm), facilitates protein migration through the membrane, but captures EVs on the membrane surface. Compared to conventional procedures, this method shows up to 65% EV recovery estimated at the RNA level (similar to 7.9 times better than U/C) and up to 83.6% protein removal (residual protein amount is approximately half of the precipitate) in similar to 30 min (similar to 9 times faster than U/C). With optimized working range of voltage and use of biochemically stable buffers, isolated EV are fully compatible with biological post-processes and assays. (C) 2016 Elsevier B.V. All rights reserved.
URI
https://www.sciencedirect.com/science/article/pii/S0925400516305731?via%3Dihubhttp://hdl.handle.net/20.500.11754/46937
ISSN
0925-4005
DOI
10.1016/j.snb.2016.04.091
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > MECHANICAL ENGINEERING(기계공학부) > Articles
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