Knockdown of hepatoma-derived growth factor-related protein-3 induces apoptosis of H1299 cells via ROS-dependent and p53-independent NF-kappa B activation
- Title
- Knockdown of hepatoma-derived growth factor-related protein-3 induces apoptosis of H1299 cells via ROS-dependent and p53-independent NF-kappa B activation
- Author
- 이수재
- Keywords
- H1299 cells; HRP-3; Myc/Noxa signaling; NF-kappa B; ROS
- Issue Date
- 2014-07
- Publisher
- Elsevier Science INC
- Citation
- Biochemical and Biophysical Research Communications, 2014, 449(4), P.471-476
- Abstract
- We previously identified hepatoma-derived growth factor-related protein-3 (HRP-3) as a radioresistant biomarker in p53 wild-type A549 cells and found that p53-dependent induction of the PUMA pathway was a critical event in regulating the radioresistant phenotype. Here, we found that HRP-3 knockdown regulates the radioresistance of p53- H1299 cells through a distinctly different molecular mechanism. HRP-3 depletion was sufficient to cause apoptosis of H1299 cells by generating substantial levels of reactive oxygen species (ROS) through inhibition of the Nrf2/HO-1 antioxidant pathway. Subsequent, ROS-dependent and p53-independent NF-kappa B activation stimulated expression of c-Myc and Noxa proteins, thereby inducing the apoptotic machinery. Our results thus extend the range of targets for the development of new drugs to treat both p53 wild-type or p53- radioresistant lung cancer cells. (C) 2014 The Authors. Published by Elsevier Inc.
- URI
- https://www.sciencedirect.com/science/article/pii/S0006291X14009024?via%3Dihub
- ISSN
- 0006-291X; 1090-2104
- DOI
- 10.1016/j.bbrc.2014.05.039
- Appears in Collections:
- COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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