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Fabrication and sensing property for conducting polymer nanowire-based biosensor for detection of immunoglobulin G

Title
Fabrication and sensing property for conducting polymer nanowire-based biosensor for detection of immunoglobulin G
Author
임동우
Keywords
Conducting polymer nanowire; Biosensor; Electrospinning; Surface immobilization
Issue Date
2014-05
Publisher
SPRINGER, VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS
Citation
RESEARCH ON CHEMICAL INTERMEDIATES, 권: 40, 호: 7, 페이지: 2565-2570
Abstract
Conducting polymers are excellent sensing materials in the design of bioanalytical sensors because of their electronic conductivity, low energy optical transitions, biocompatibility, and room temperature operation. Among them, Polypyrrole (Ppy) is one of the most extensively used conducting polymers because of a number of properties such as redox activity, rapid electron transfer, and ability to link a variety of biomolecules to pyrrole groups by chemical treatment. In this study, Ppy nanowires were synthesized by an electrospinning method. The nanowires were prepared from a solution mixture of Ppy and poly(ethylene oxide). The method of detection in such a device is based on the selective binding of antigen onto an antibody that is covalently attached to the nanowires. Thus, anti-IgG was immobilized on Ppy nanowires using an EDC {[N-(3-dimethyl aminopropyl)-N-2-ethylcarbodiimide hydrochloride]}-NHS(N-hydrosuccinimide) modified technique. Fluorescence images of BSA-FITC (fluorescein isothiocyanate labeling of bovine serum albumin) conjugation demonstrated that antibody was functionalized on the Ppy nanowires without non-specific binding and facilitated selective detection of antigen. Current-voltage (I-V) characterization was used to monitor the change in the conductivity of nanowires while the specific binding interaction occurred. These results of electrical properties enable Ppy nanowire-based biosensors to detect biomolecules in real-time.
URI
https://link.springer.com/article/10.1007%2Fs11164-014-1669-7http://hdl.handle.net/20.500.11754/46149
ISSN
0922-6168; 1568-5675
DOI
10.1007/s11164-014-1669-7
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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