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Hierarchical Silicon Nanospikes Membrane for Rapid and High-Throughput Mechanical Cell Lysis

Title
Hierarchical Silicon Nanospikes Membrane for Rapid and High-Throughput Mechanical Cell Lysis
Author
소홍윤
Keywords
hierarchical structure; porous silicon membrane; ultrasharp nanospikes; electrochemical etching; cell lysis; point-of-care diagnostics
Issue Date
2014-05
Publisher
AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA
Citation
ACS APPLIED MATERIALS & INTERFACES, 권: 6, 호: 10, 페이지: 6993-6997
Abstract
This letter reports an efficient and compatible silicon membrane combining the physical properties of nanospikes and microchannel arrays for mechanical cell lysis. This hierarchical silicon nanospikes membrane was created to mechanically disrupt cells for a rapid process with high throughput, and it can be assembled with commercial syringe filter holders. The membrane was fabricated by photoelectrochemical overetching to form ultrasharp nanospikes in situ along the edges of the microchannel arrays. The intracellular protein and nucleic acid concentrations obtained using the proposed membrane within a short period of time were quantitatively higher than those obtained by routine, conventional acoustic and chemical lysis methods.
URI
https://pubs.acs.org/doi/10.1021/am501221bhttp://hdl.handle.net/20.500.11754/45884
ISSN
1944-8244
DOI
10.1021/am501221b
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > MECHANICAL ENGINEERING(기계공학부) > Articles
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