Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 황승용 | - |
dc.date.accessioned | 2018-03-12T06:55:50Z | - |
dc.date.available | 2018-03-12T06:55:50Z | - |
dc.date.issued | 2013-06 | - |
dc.identifier.citation | Biochip Journal, June 2013, 7(2), p.164-172 | en_US |
dc.identifier.issn | 2092-7843 | - |
dc.identifier.issn | 1976-0280 | - |
dc.identifier.uri | https://link.springer.com/article/10.1007/s13206-013-7210-z | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/45475 | - |
dc.description.abstract | A protease is any enzyme that catalyzes the hydrolysis of proteins into smaller peptide fragments and amino acids, a process known as proteolysis. They are involved in a multitude of normal biological processes as well as in diseases, including cancer, stroke and infections. Here we present a microfluidicbased assay system to detect proteolytic activity using fluorescence resonance energy transfer (FRET) by quantum dot (QD)-peptide conjugates immobilized on microbeads. As an energy donor, QD was immobilized on the microbead surface by the avidin-biotin interaction. As an energy acceptor, the fluorophorelabeled peptide was then associated with QD, thus quenching the photoluminescence (PL) of the QD. The functionalized microbeads were introduced into the microbiochip and captured by a micropillar in the reaction chamber. In the presence of matrix metalloprotease-7 (MMP-7) as a model protease, the PL of QD quenched by fluorophore was recovered due to the proteolytic activity of MMP-7 in the fabricated microbiochip. Moreover, the FRET efficiency induced by MMP-7 was linearly dependent on the logarithmic concentration of MMP-7. This technology is not limited to sensing MMP-7, but could be used to monitor other protease activities (Schematic diagram). | en_US |
dc.description.sponsorship | This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (20090074380,20110005094). | en_US |
dc.language.iso | en | en_US |
dc.publisher | SpringerOpen | en_US |
dc.subject | Bead-based assay | en_US |
dc.subject | Enzyme assay | en_US |
dc.subject | Fluorescence Resonance Energy Transfer (FRET) | en_US |
dc.subject | Matrix Metalloproteinase 7 | en_US |
dc.subject | Microbiochip | en_US |
dc.subject | Quantum Dot (QD) | en_US |
dc.title | Development of microbiochip for detection of metalloproteinase 7 using fluorescence resonance energy transfer | en_US |
dc.type | Article | en_US |
dc.relation.no | 2 | - |
dc.relation.volume | 7 | - |
dc.identifier.doi | 10.1007/s13206-013-7210-z | - |
dc.relation.page | 164-172 | - |
dc.relation.journal | BIOCHIP JOURNAL | - |
dc.contributor.googleauthor | Lee, S.Y. | - |
dc.contributor.googleauthor | Park, C | - |
dc.contributor.googleauthor | Jeong, J.-S | - |
dc.contributor.googleauthor | Ahn, J.J. | - |
dc.contributor.googleauthor | Hwang, S.Y. | - |
dc.contributor.googleauthor | Han, B. | - |
dc.contributor.googleauthor | Ha, S.-M | - |
dc.contributor.googleauthor | Ahn, Y. | - |
dc.relation.code | 2013001127 | - |
dc.sector.campus | S | - |
dc.sector.daehak | GRADUATE SCHOOL[S] | - |
dc.sector.department | DEPARTMENT OF BIONANOTECHNOLOGY | - |
dc.identifier.pid | syhwang | - |
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