Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이수재 | - |
dc.date.accessioned | 2018-03-10T05:48:49Z | - |
dc.date.available | 2018-03-10T05:48:49Z | - |
dc.date.issued | 2013-10 | - |
dc.identifier.citation | INTERNATIONAL JOURNAL OF ONCOLOGY, 2013, 43(4), P.1260-1268 | en_US |
dc.identifier.issn | 1019-6439 | - |
dc.identifier.issn | 1791-2423 | - |
dc.identifier.uri | http://search.proquest.com/docview/1932330661/fulltextPDF/A977011150FE4610PQ/1?accountid=11283 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/44754 | - |
dc.description.abstract | Cells resembling bone marrow mesenchymal stem cells (BM-MSCs) have been isolated from glioma specimens; however, little is known about the existence of mesenchymal stem-like cells (MSLCs) in meningioma. Here, we hypothesized that cells similar to BM-MSCs exist in meningioma specimens and sought to investigate whether these putative meningioma stroma MSLCs (MS-MSLCs) could be isolated. To this end, we cultured fresh meningioma specimens using the same protocols as used previously to isolate BM-MSC. Cultured cells were analyzed for surface markers associated with BM-MSCs by fluorescence-activated cell sorting (FACS) and candidate cells were exposed to mesenchymal differentiation conditions. Possible locations of MS-MSLCs were determined by immunohistochemical analysis of sections of meningioma specimens. Spindle-shaped and, adherent cells similar to BM-MSCs were isolated in 2 of 20 meningioma specimens. FACS analysis showed that the surface markers of MS-MSLCs were similar to those of BM-MSCs and the chosen cells demonstrated an ability to differentiate into osteogenic, adipogenic and chondrogenic cells. The tumorigenicity of MS-MSLCs was tested by injection of these cells into the brain of athymic nude mice; no tumors were subsequently discovered. Immunohistochemical analyses indicated that CD105(+) cells were closely associated with endothelial cells and pericytes in meningioma specimens. Our results established for the first time that cells similar to BM-MSCs exist in meningioma specimens. These cells, termed MS-MSLCs, could be one component of the meningioma cellular microenvironment. | en_US |
dc.description.sponsorship | This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (NRF-2013R1A1A2006427) and a grant from the National R&D Program for Cancer Control, Ministry for Health, Welfare and Family Affairs, Republic of Korea (1020340). | en_US |
dc.language.iso | en | en_US |
dc.publisher | Spandidos Publications | en_US |
dc.subject | meningioma | en_US |
dc.subject | meningioma stroma | en_US |
dc.subject | mesenchymal stem-like cells | en_US |
dc.subject | microenvironment | en_US |
dc.subject | perivascular area | en_US |
dc.subject | CENTRAL-NERVOUS-SYSTEM | en_US |
dc.subject | CANCER METASTASIS | en_US |
dc.subject | GLIOMA SPECIMENS | en_US |
dc.subject | ARACHNOID CELLS | en_US |
dc.subject | BREAST-CANCER | en_US |
dc.subject | BRAIN CANCER | en_US |
dc.subject | TUMOR-CELLS | en_US |
dc.subject | BONE-MARROW | en_US |
dc.subject | STROMA | en_US |
dc.subject | MICROENVIRONMENT | en_US |
dc.title | Isolation of mesenchymal stem-like cells in meningioma specimens | en_US |
dc.type | Article | en_US |
dc.relation.volume | 43 | - |
dc.identifier.doi | 10.3892/ijo.2013.2053 | - |
dc.relation.page | 1260-1268 | - |
dc.relation.journal | INTERNATIONAL JOURNAL OF ONCOLOGY | - |
dc.contributor.googleauthor | Lim, Hyo-Yeol | - |
dc.contributor.googleauthor | Kim, Kyung Min | - |
dc.contributor.googleauthor | Kim, Bo Kyung | - |
dc.contributor.googleauthor | Shim, Jin-Kyoung | - |
dc.contributor.googleauthor | Lee, Ji-Hyun | - |
dc.contributor.googleauthor | Huh, Yong-Min | - |
dc.contributor.googleauthor | Kim, Se-Hoon | - |
dc.contributor.googleauthor | Kim, Eui-Hyun | - |
dc.contributor.googleauthor | Park, Eun-Kyung | - |
dc.contributor.googleauthor | Lee, Su-Jae | - |
dc.relation.code | 2013010365 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF LIFE SCIENCE | - |
dc.identifier.pid | sj0420 | - |
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