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Transformation of somatic cells into stem cell-like cells under a stromal niche

Title
Transformation of somatic cells into stem cell-like cells under a stromal niche
Author
김계성
Keywords
genetic and cellular plasticity; colony-forming fibroblasts; reprogramming; pluripotency; microenvironment
Issue Date
2013-07
Publisher
FEDERATION OF AMERICAN SOC OF EXP BIOLOGY
Citation
FASEB JOURNAL, 권: 27, 호: 7, 페이지: 2644-2656
Abstract
To study the genomic plasticity of somatic cells without ectopic genetic manipulation, we cultured mouse fibroblasts with ovarian cells, embryonic fibroblasts of different strains, and parthenogenetic embryonic stem cells (ESCs). Of 41 trials, cell aggregation resembling nascent ESC colony from inner cell mass was detected in 9 cases (22%), and 6 cases (67%) yielded fibroblast-derived colonies with ESC morphology. Cells used in coculture provided the critical (P=0.0061) inducing factor for the aggregation. These colony-forming fibroblasts (CFFs) showed similar characteristics to those in ESCs and induced pluripotent stem cells (iPSCs), including pluripotency gene expression, in vitro differentiation, and teratoma formation. Furthermore, CFFs produced somatic chimera, although none showed germline chimerism. CFFs had a tetraploid-like karyotype, and their imprinting patterns differed from parthenogenetic ESCs, thereby confirming their nongermline transmissibility. We observed dysregulation of cell cycle-related proteins, as well as both homologous and heterologous recombination of genomic single-nucleotide polymorphisms in CFFs. Our observations provide information on somatic cell plasticity, resulting in stemness or tumorigenesis, regardless of colony-forming cell progenitors in the fibroblast population. The plasticity of somatic genomes under environmental influences, as well as acquisition of pluripotency by cell fusion, is also implicated.Lee, S. T., Gong, S. P., Yum, K. E., Lee, E. J., Lee, C. H., Choi, J. H., Kim, D. Y., Han, H., Kim, K.-S., Hysolli, E., Ahn, J. Y., Park, I.-H., Han, J. Y., Jeong, J.-W., Lim, J. M. Transformation of somatic cells into stem cell-like cells under a stromal niche.
URI
http://www.fasebj.org/doi/10.1096/fj.12-223065http://hdl.handle.net/20.500.11754/44285
ISSN
0892-6638; 1530-6860
DOI
10.1096/fj.12-223065
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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