Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 최종훈 | - |
dc.date.accessioned | 2018-03-09T05:30:35Z | - |
dc.date.available | 2018-03-09T05:30:35Z | - |
dc.date.issued | 2013-07 | - |
dc.identifier.citation | Nanotechnology,2013,24(28),p1-7 | en_US |
dc.identifier.issn | 0957-4484 | - |
dc.identifier.uri | http://iopscience.iop.org/article/10.1088/0957-4484/24/28/285103 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/44181 | - |
dc.description.abstract | The field of biomarker quantification has experienced a growth parallel to the discovery of new materials. In this paper, we propose an innovative system for the separation and quantification of biomarkers using a simple magnetic bead (MB)-quantum dot (QD) sandwich assay. The basis of the system lies in the interaction between histidine residues on protein G and Ni ions on QDs, and the use of imidazole to selectively detach QDs bound to target biomarkers, in effect enumerating the absolute number of biomarker units. We used C-reactive protein (CRP) as a proof-of-concept and demonstrated a detection sensitivity of 82.5 fmoles in 50 mu 1 of sample volume, a commonly used analytical volume (e.g. ELISA). Although CRP was used as a model to conduct this study, the sensitivity and simplicity of this detachable system make it a viable approach in the quantification of other target analytes. | en_US |
dc.description.sponsorship | The authors would like to gratefully acknowledge the financial support provided by the Korea Institute of Science and Technology (KIST Institutional Project #2E23720 and K-GRL #2Z03720). We would also like to thank Professor Chul Sung Kim of Kookmin University for his valuable expertise and for kindly facilitating the use of a vibrating sample magnetometer. | en_US |
dc.language.iso | en | en_US |
dc.publisher | IOP PUBLISHING LTD, TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND | en_US |
dc.subject | C-REACTIVE PROTEIN | en_US |
dc.subject | MAGNETIC MICROBEADS | en_US |
dc.subject | IMMUNOASSAYS | en_US |
dc.subject | CELLS | en_US |
dc.subject | HUMIDITY | en_US |
dc.subject | DISEASE | en_US |
dc.subject | DESIGN | en_US |
dc.subject | CANCER | en_US |
dc.title | Harnessing immunomagnetic separation and quantum dot-based quantification capacities for the enumeration of absolute levels of biomarker | en_US |
dc.type | Article | en_US |
dc.relation.no | 28 | - |
dc.relation.volume | 24 | - |
dc.identifier.doi | 10.1088/0957-4484/24/28/285103 | - |
dc.relation.page | 103-110 | - |
dc.relation.journal | NANOTECHNOLOGY | - |
dc.contributor.googleauthor | Park, Ho-Young | - |
dc.contributor.googleauthor | Hwang, Mintai P. | - |
dc.contributor.googleauthor | Lee, Jong-Wook | - |
dc.contributor.googleauthor | Choi, Jong-Hoon | - |
dc.contributor.googleauthor | Lee, Kwan-Hyi | - |
dc.relation.code | 2013011387 | - |
dc.sector.campus | S | - |
dc.sector.daehak | GRADUATE SCHOOL[S] | - |
dc.sector.department | DEPARTMENT OF BIONANOTECHNOLOGY | - |
dc.identifier.pid | jonghchoi | - |
dc.identifier.researcherID | 55682949300 | - |
dc.identifier.orcid | 0000-0003-3554-7033 | - |
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