Detection of PIWI and piRNAs in the mitochondria of mammalian cancer cells
- Title
- Detection of PIWI and piRNAs in the mitochondria of mammalian cancer cells
- Author
- 노미나
- Keywords
- Small RNA-Seq; Bioinformatics; Genomics; Sequence analysis; piRNA; Piwi; Mitochondria; Non-coding RNAs
- Issue Date
- 2014-03
- Publisher
- Elsevier Science INC
- Citation
- Biochemical and Biophysical Research Communications, 2014, 446(1), P.218-223
- Abstract
- Piwi-interacting RNAs (piRNAs) are 26-31 nt small noncoding RNAs that are processed from their longer precursor transcripts by Piwi proteins. Localization of Piwi and piRNA has been reported mostly in nucleus and cytoplasm of higher eukaryotes germ-line cells, where it is believed that known piRNA sequences are located in repeat regions of nuclear genome in germ-line cells. However, localization of PIWI and piRNA in mammalian somatic cell mitochondria yet remains largely unknown. We identified 29 piRNA sequence alignments from various regions of the human mitochondrial genome. Twelve out 29 piRNA sequences matched stem-loop fragment sequences of seven distinct tRNAs. We observed their actual expression in mitochondria subcellular fractions by inspecting mitochondrial-specific small RNA-Seq datasets. Of interest, the majority of the 29 piRNAs overlapped with multiple longer transcripts (expressed sequence tags) that are unique to the human mitochondrial genome. The presence of mature piRNAs in mitochondria was detected by qRT-PCR of mitochondrial subcellular RNAs. Further validation showed detection of Piwi by colocalization using anti-Piwil1 and mitochondria organelle-specific protein antibodies. (c) 2014 The Authors. Published by Elsevier Inc.
- URI
- https://www.sciencedirect.com/science/article/pii/S0006291X14003830?via%3Dihubhttp://hdl.handle.net/20.500.11754/44136
- ISSN
- 0006-291X; 1090-2104
- DOI
- 10.1016/j.bbrc.2014.02.112
- Appears in Collections:
- COLLEGE OF ENGINEERING[S](공과대학) > COMPUTER SCIENCE(컴퓨터소프트웨어학부) > Articles
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