Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 원영웅 | - |
dc.date.accessioned | 2018-03-09T05:00:35Z | - |
dc.date.available | 2018-03-09T05:00:35Z | - |
dc.date.issued | 2013-04 | - |
dc.identifier.citation | Tumor Biology, April 2013, 34(2), P.875-885 | en_US |
dc.identifier.issn | 1010-4283 | - |
dc.identifier.uri | https://link.springer.com/article/10.1007%2Fs13277-012-0621-y | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/44118 | - |
dc.description.abstract | Extracts of the whole herb of Artemisia asiatica Nakai (Asteraceae) are used in traditional oriental medicine to treat inflammation. Eupatilin (5,7-dihydroxy-3',4',6-trimethoxyflavone) is one of the pharmacologically active components found in A. asiatica, and has been shown to possess anti-tumoral effects in some malignancies, including gastric cancer. However, its anti-metastatic effect in gastric cancer is hardly known. In this study, anti-metastatic effect of eupatilin was investigated in the human gastric cancer cell line, MKN-1. Eupatilin inhibited MKN-1 growth in a dose- and a time-dependent manner, and induced apoptosis with a concomitant increase of caspase-3 activity. ELISA demonstrated that release of pro-inflammatory cytokines (IL-1 beta, TNF-alpha, IL-6, and IL-8) was significantly reduced by eupatilin. And p-AKT and p-ERK (p44/42) was reduced. Expression level of beta-catenin and integrin was reduced and p-GSK beta was increased. In transcription reporter system, the activity of the transcriptional factor, NF-kappa B, was reduced by eupatilin and the expression of p65 was down-regulated when MKN-1 cells were treated with eupatilin. Moreover, a zymography study revealed that this reduction in invasive potential resulted from a reduction in type IV collagenolytic (gelatinolytic) activity. The expressions of metalloproteinases (MMP-2 and MMP-9) were also reduced in MKN-1 cells treated with eupatilin. In vitro invasion assay, eupatilin inhibited MKN-1 penetrating reconstituted basement membrane barriers. These results suggest that eupatilin inhibits the MKN-1 gastric cancer cell proliferation via activation of caspase-3 and the metastatic potential of gastric cancer cells via down-regulation of NF-kappa B activity followed by reduction of pro-inflammatory cytokine-mediated MMPs expressions. | en_US |
dc.description.sponsorship | Hanyang University | en_US |
dc.language.iso | en | en_US |
dc.publisher | Springer | en_US |
dc.subject | Growth inhibition | en_US |
dc.subject | Gastric cancer | en_US |
dc.subject | Eupatilin | en_US |
dc.subject | Metastasis | en_US |
dc.subject | Invasion | en_US |
dc.title | Inhibitory effects of eupatilin on tumor invasion of human gastric cancer MKN-1 cells | en_US |
dc.type | Article | en_US |
dc.relation.no | 2 | - |
dc.relation.volume | 34 | - |
dc.identifier.doi | 10.1007/s13277-012-0621-y | - |
dc.relation.page | 875-885 | - |
dc.relation.journal | TUMOR BIOLOGY | - |
dc.contributor.googleauthor | Park, Byeong Bae | - |
dc.contributor.googleauthor | Yoon, Jin Sun | - |
dc.contributor.googleauthor | Kim, Eun Shil | - |
dc.contributor.googleauthor | Choi, Jiyoung | - |
dc.contributor.googleauthor | Won, Young Woong | - |
dc.contributor.googleauthor | Choi, Jung Hye | - |
dc.contributor.googleauthor | Lee, Young Yiul | - |
dc.relation.code | 2013012256 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | wonhero | - |
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