Butyl paraben-induced changes in DNA methylation in rat epididymal spermatozoa
- Title
- Butyl paraben-induced changes in DNA methylation in rat epididymal spermatozoa
- Author
- 계명찬
- Keywords
- Butyl paraben; DNA methylation; epididymal spermatozoa; rat
- Issue Date
- 2012-04
- Publisher
- WILEY-BLACKWELL
- Citation
- Andrologia, May 2012, 44(1), P.187-193
- Abstract
- Parabens have been shown to affect male rodent reproductive parameters, including testosterone levels and sperm production. In this study, we examined the effect of long-term exposure to butyl paraben (BP) on rat epididymal sperm DNA methylation. Adult male rats were exposed to BP (0, 10, 100 and 1000 mg kg-1 per day) according to OECD TG407 for a repeated 28-day oral toxicity study. Sperm DNA methylation was examined by differential display random amplification of polymorphic DNA (RAPD) following methylation-specific restriction digestion of DNA. Among the 57 RAPD amplicons, six were methylation specific. Of these, five amplicons increased by 1.4- to 3.8-fold in epididymal sperm DNA at testing dose of BP. This indicates that BP can cause DNA hypermethylation in germ cells from the mitotic through post-meiotic stage in adult rat testes. To our knowledge, this is the first report on the epigenetic modification of sperm DNA by parabens.
- URI
- http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0272.2011.01162.x/abstracthttp://hdl.handle.net/20.500.11754/43534
- ISSN
- 0303-4569; 1439-0272
- DOI
- 10.1111/j.1439-0272.2011.01162.x
- Appears in Collections:
- COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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