Low doses of PEG-coated gold nanoparticles sensitize solid tumors to cold plasma by blocking the PI3K/AKT-driven signaling axis to suppress cellular transformation by inhibiting growth and EMT
- Title
- Low doses of PEG-coated gold nanoparticles sensitize solid tumors to cold plasma by blocking the PI3K/AKT-driven signaling axis to suppress cellular transformation by inhibiting growth and EMT
- Author
- KAUSHIK, NEHA
- Keywords
- Cold plasma; Gold nanoparticles; Solid cancers; Stemness; Glioma-like stem cells; Epithelial-mesenchymal transition
- Issue Date
- 2016-02
- Publisher
- ELSEVIER SCI LTD
- Citation
- BIOMATERIALS, v.87, page.118-130
- Abstract
- Metastasis, the primary cause of tumor cell transformation, is often activated during cancer invasion and progression and is associated with poor therapeutic outcomes. The effects of combined treatments that included PEG-coated gold nanoparticles (GNP) and cold plasma on epithelialemesenchymal transition (EMT) and the maintenance of cancer stem cells (CSC) have not been described so far. Here, we report that co-treatment with GNP and cold plasma inhibited proliferation in cancer cells by abolishing the activation of the PI3K/AKT signaling axis. In addition, co-treatment reversed EMT in solid tumor cells by reducing the secretion of a number of proteins, resulting in the upregulation of epithelial markers such as E-cadherin along with down-regulation of N-Cadherin, Slug and Zeb-1. The inhibition of the PI3K/AKT pathway and the reversal of EMT by co-treatment prevented tumor cells growth in solid tumors. Furthermore, we show that GNP and plasma also suppresses tumor growth by decreasing mesenchymal markers in tumor xenograft mice models. Importantly, co-treatment resulted in a substantial decrease in sphere formation and the self-renewal capacity of glioma-like stem cells. Together, these results indicate a direct link between a decrease of EMT and an increase in cell death in solid tumors following co- treatment with cold plasma and GNP.
- URI
- https://www.sciencedirect.com/science/article/pii/S0142961216001289?via%3Dihubhttp://hdl.handle.net/20.500.11754/42960
- ISSN
- 0142-9612; 1878-5905
- DOI
- 10.1016/j.biomaterials.2016.02.014
- Appears in Collections:
- RESEARCH INSTITUTE[S](부설연구소) > THE RESEARCH INSTITUTE FOR NATURAL SCIENCES(자연과학연구소) > Articles
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