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dc.contributor.author이근용-
dc.date.accessioned2018-03-01T04:14:42Z-
dc.date.available2018-03-01T04:14:42Z-
dc.date.issued2013-01-
dc.identifier.citationCARBOHYDRATE POLYMERS 권: 92 호: 1 페이지: 57-62en_US
dc.identifier.issn0144-8617-
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0144861712009083?via%3Dihub-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/41449-
dc.description.abstractChitosan-based nanoparticles have been widely used as a carrier for gene delivery due to their low toxicity and the positively charged amino groups in chitosan. In this study, we hypothesized that introduction of nonaarginine to chitosan could improve its ability to form a complex with siRNA, as well as enhance the cellular uptake and transfection efficiency of chitosan-based nanoparticles. To this end, a peptide with nine repeating arginine residues was chemically coupled to the chitosan backbone, and various characteristics of nonaarginine-chitosan/siRNA nanoparticles were investigated. The mean diameter and zeta potential of the nonaarginine-chitosan/siRNA nanoparticles were dependent on the amount of nonaarginine conjugated to chitosan. Nonaarginine-modified chitosan/siRNA nanoparticles demonstrated enhanced cellular association and transfection efficiency in vitro, while maintaining a low level of cytotoxicity. In conclusion, nonaarginine-modified chitosan should be considered a potential carrier for various gene delivery applications. (C) 2012 Elsevier Ltd. All rights reserved.en_US
dc.description.sponsorshipThis research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (20120004980).en_US
dc.language.isoenen_US
dc.publisherELSEVIER SCI LTD, THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDen_US
dc.subjectChitosanen_US
dc.subjectNonaarginineen_US
dc.subjectsiRNAen_US
dc.subjectGene deliveryen_US
dc.subjectGENE DELIVERYen_US
dc.subjectRNA INTERFERENCEen_US
dc.subjectARGININEen_US
dc.subjectTHERAPYen_US
dc.subjectCHITINen_US
dc.subjectDNAen_US
dc.subjectPOLYMERSen_US
dc.subjectSYSTEMSen_US
dc.subjectMICEen_US
dc.titlePreparation and characterization of nonaarginine-modified chitosan nanoparticles for siRNA deliveryen_US
dc.typeArticleen_US
dc.relation.no1-
dc.relation.volume92-
dc.identifier.doi10.1016/j.carbpol.2012.08.116-
dc.relation.page57-62-
dc.relation.journalCARBOHYDRATE POLYMERS-
dc.contributor.googleauthorPark, So yeon-
dc.contributor.googleauthorJeong, Eun Ju-
dc.contributor.googleauthorLee, Jang wook-
dc.contributor.googleauthorRhim, Tai youn-
dc.contributor.googleauthorLee, Sang Kyung-
dc.contributor.googleauthorLee, Kuen Yong-
dc.relation.code2012201694-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF ENGINEERING[S]-
dc.sector.departmentDEPARTMENT OF BIOENGINEERING-
dc.identifier.pidleeky-
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COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
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