Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 윤채옥 | - |
dc.date.accessioned | 2018-03-01T02:19:14Z | - |
dc.date.available | 2018-03-01T02:19:14Z | - |
dc.date.issued | 2012-09 | - |
dc.identifier.citation | PLASTIC AND RECONSTRUCTIVE SURGERY,Vol130,No3,p407E-417E | en_US |
dc.identifier.issn | 0032-1052 | - |
dc.identifier.uri | http://ovidsp.tx.ovid.com/sp-3.28.0a/ovidweb.cgi?QS2=434f4e1a73d37e8c6dbab9f901264ada47b2e68359d387495f2efc7905d72b9da953941d39d07d09fa8981bfd1bb055aefeae5c08fad6a527a234f1181474aa9b1026117a39a519b9cd6dc15db47cb25b28bb2421c21e6d92d94169134ad6eee276c4ef16d159750e42da3f399ef017df3b2b93c0cec312c977467fe1bbf1f0bdfebd4105392e2b99c5f9c98fdf60ae8 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/41410 | - |
dc.description.abstract | Background: The hormone relaxin has been shown to affect the extracellular matrix by inhibiting collagen synthesis and expression in fibroblasts stimulated with a profibrotic agent. It also increases matrix metalloproteinase (MMP) expression. To investigate its effect on expression of collagen and MMPs in keloid fibroblasts and human dermal fibroblasts, the authors introduced a relaxin-expressing adenovirus (dE1-RGD/lacZ/RLX) into a human dermal fibroblast cell line and keloid fibroblasts. Methods: Both fibroblasts were infected with dE1-RGD/lacZ/RLX or control virus, and protein levels of relaxin and secreted transforming growth factor (TGF)-beta 1 were assessed by enzyme-linked immunosorbent assay, and mRNA levels o 0000005E f collagen type I, collagen type III, MMP-1, and MMP-3 were assessed by real-time reverse-tran 00001BE4 scriptase polymerase chain reaction and enzyme-linked immunosorbent assay. Expression of Smad3 and phosphorylated Smad3 was also examined, and relaxin's effect on Smad2/3 complex localization was evaluated. Results: When human dermal fibroblasts and keloid fibroblasts were transduced with dE1-RGD/lacZ/RLX or dE1-RGD/lacZ (control), mRNA expression of type I and type III collagen was markedly decreased by relaxin regardless of TGF-beta (10 ng/ml) treatment. Expression of Smad3 and phosphorylated Smad3 was reduced in keloid fibroblasts and decreased translocation of Smad 2/3 complex from cytosols to the nucleus of the human dermal fibroblasts with TGF-beta after dE1-RGD/lacZ/RLX transduction, suggesting that relaxin reduces collagen synthesis by blocking TGF-beta signaling. Analyses revealed that MMP-1 and MMP-3 expression were significantly increased in human dermal fibroblasts and keloid fibroblasts after dE1-RGD/lacZ/RLX transduction. Conclusion: These results suggest that the antifibrotic effect of relaxin-expressing adenovirus may have therapeutic effects on keloids. (Plast. Reconstr. Surg. 130: 407e, 2012.) | en_US |
dc.description.sponsorship | This work was supported by grants from the Ministry of Knowledge Economy (10030051, to C.O.Y.), the Korea Science and Engineering Foundation (R15-2004-024-02001-0 and 2009K001644, to C.O.Y.), the Korea Food and Drug Administration (10172-332, to C.O.Y.); by a National Research Foundation of Korea grant funded by the Korea government (Ministry o 00000D8C f Education, Science and Technology) (2011-0022012, to W.J.L.); and by a National Research Foundation of Korea grant funded by the Korean government (Ministry of Education, Science and Technology) (2010-0010475, to J.H.L.) | en_US |
dc.language.iso | en | en_US |
dc.publisher | LIPPINCOTT WILLIAMS & WILKINS | en_US |
dc.subject | REVERSES CARDIAC FIBROSIS | en_US |
dc.subject | GENE-THERAPY | en_US |
dc.subject | HYPERTROPHIC SCARS | en_US |
dc.subject | RENAL-DISEASE | en_US |
dc.subject | PROLIFERATION | en_US |
dc.subject | INHIBITION | en_US |
dc.subject | IVO | en_US |
dc.subject | PROGRESSION | en_US |
dc.subject | SECRETION | en_US |
dc.subject | MIGRATION | en_US |
dc.title | Relaxin-Expressing Adenovirus Decreases Collagen Synthesis and Up-Regulates Matrix Metalloproteinase Expression in Keloid Fibroblasts: In Vitro Experiments | en_US |
dc.type | Article | en_US |
dc.relation.no | 3 | - |
dc.relation.volume | 130 | - |
dc.identifier.doi | 10.1097/PRS.0b013e31825dbf56 | - |
dc.relation.page | 407-417 | - |
dc.relation.journal | PLASTIC AND RECONSTRUCTIVE SURGERY | - |
dc.contributor.googleauthor | Lee, Won Jai | - |
dc.contributor.googleauthor | Choi, Il-Kyu | - |
dc.contributor.googleauthor | Lee, Ju Hee | - |
dc.contributor.googleauthor | Lee, Jung-Sun | - |
dc.contributor.googleauthor | Kim, Yong Oock | - |
dc.contributor.googleauthor | Rah, Dong Kyun | - |
dc.contributor.googleauthor | Yun, Chae-Ok | - |
dc.relation.code | 2012207692 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | chaeok | - |
dc.identifier.researcherID | P-3698-2015 | - |
dc.identifier.orcid | http://orcid.org/0000-0002-9466-4531 | - |
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