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In Vivo Differentiation of Human Amniotic Epithelial Cells Into Cardiomyocyte-Like Cells and Cell Transplantation Effect on Myocardial Infarction in Rats: Comparison With Cord Blood and Adipose Tissue-Derived Mesenchymal Stem Cells

Title
In Vivo Differentiation of Human Amniotic Epithelial Cells Into Cardiomyocyte-Like Cells and Cell Transplantation Effect on Myocardial Infarction in Rats: Comparison With Cord Blood and Adipose Tissue-Derived Mesenchymal Stem Cells
Author
이영열
Keywords
Human amniotic epithelial cells; Cardiomyocytes; Differentiation; Myocardial infarction; LEFT-VENTRICULAR FUNCTION; IMPROVES CARDIAC-FUNCTION; SKELETAL MYOBLASTS; THERAPY; REPAIR; MODEL; HEART; ANGIOGENESIS; REGENERATION; MULTIPOTENT
Issue Date
2012-08
Publisher
COGNIZANT COMMUNICATION CORP, 18 PEEKSKILL HOLLOW RD, PO BOX 37, PUTNAM VALLEY, NY 10579 USA
Citation
CELL TRANSPLANTATION, 21, 8, 1687~1783
Abstract
Human amniotic epithelial cells (h-AECs), which have various merits as a cell source for cell therapy, are known to differentiate into cardiomyocytes in vitro. However, the ability of h-AECs to differentiate into cardiomyocytes in vivo and their cell transplantation effects on myocardial infarction are still unknown. In this study, we assessed whether h-AECs could differentiate into cardiomyocytes in vivo and whether h-AECs transplantation can decrease infarct size and improve cardiac function, in comparison to transplantation of cord blood-derived mesenchymal stem cells (MSCs) or adipose tissue-derived MSCs. For our study, we injected h-AECs, cord blood-derived MSCs, adipose tissue-derived MSCs, and saline into areas of myocardial infarction in athymic nude rats. After 4 weeks, 3% of the surviving h-AECs expressed myosin heavy chain, a marker specific to the myocardium. Compared with the saline group, all cell-implanted groups showed a higher ejection fraction, lower infarct area by positron emission tomography and histology, and more abundant myocardial gene and protein expression in the infarct area. We showed that h-AECs can differentiate into cardiomyocyte-like cells, decrease infarct size, and improve cardiac function in vivo. The beneficial effects of h-AECs were comparable to those of cord blood and adipose tissue-derived MSCs. These results support the need for further studies of h-AECs as a cell source for myocardial regeneration due to their plentiful availability, low immunity, and lack of ethical issues related to their use.
URI
http://journals.sagepub.com/doi/10.3727/096368912X653039http://hdl.handle.net/20.500.11754/41346
ISSN
0963-6897
DOI
10.3727/096368912X653039
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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