Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 정승준 | - |
dc.date.accessioned | 2018-02-28T06:47:58Z | - |
dc.date.available | 2018-02-28T06:47:58Z | - |
dc.date.issued | 2012-09 | - |
dc.identifier.citation | Plos One, Jule 2012, 7(7), e39715 | en_US |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0039715 | - |
dc.description.abstract | Homogeneous culture of neural precursor cells (NPCs) derived from human pluripotent stem cells (hPSCs) would provide a powerful tool for biomedical applications. However, previous efforts to expand mechanically dissected neural rosettes for cultivation of NPCs remain concerns regarding non-neural cell contamination. In addition, several attempts to purify NPCs using cell surface markers have not demonstrated the expansion capability of the sorted cells. In the present study, we show that polysialic acid-neural cell adhesion molecule (PSA-NCAM) is detected in neural rosette cells derived from hPSCs, and employ PSA-NCAM as a marker for purifying expandable primitive NPCs from the neural rosettes. PSA-NCAM-positive NPCs (termed hNPC(PSA-NCAM+)) were isolated from the heterogeneous cell population of mechanically harvested neural rosettes using magnetic-based cell sorting. The hNPC(PSA-NCAM+) extensively expressed neural markers such as Sox1, Sox2, Nestin, and Musashi-1 (80 similar to 98% of the total cells) and were propagated for multiple passages while retaining their primitive characteristics in our culture condition. Interestingly, PSA-NCAM-negative cells largely exhibited characteristics of neural crest cells. The hNPC(PSA-NCAM+) showed multipotency and responsiveness to instructive cues towards region-specific neuronal subtypes in vitro. When transplanted into the rat striatum, hNPC(PSA-NCAM+) differentiated into neurons, astrocytes, and oligodendrocytes without particular signs of tumorigenesis. Furthermore, Ki67-positive proliferating cells and non-neural lineage cells were rarely detected in the grafts of hNPC(PSA-NCAM+) compared to those of neural rosette cells. Our results suggest that PSA-NCAM-mediated cell isolation provides a highly expandable population of pure primitive NPCs from hPSCs that will lend themselves as a promising strategy for drug screening and cell therapy for neurodegenerative disorders. | en_US |
dc.description.sponsorship | This work was supported by a grant from the Stem Cell Research Center of the 21st Century Frontier Research Program (SC1110), National Research Foundation (NRF 2010-0020408 and 2010-0020353) funded by the Ministry of Education, Science and Technology, Korea. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Public Library Science | en_US |
dc.subject | HUMAN EMBRYONIC STEM | en_US |
dc.subject | LONG-TERM PROLIFERATION | en_US |
dc.subject | DOPAMINE NEURONS | en_US |
dc.subject | LINEAGE SELECTION | en_US |
dc.subject | POLYSIALIC ACID | en_US |
dc.subject | NERVOUS-SYSTEM | en_US |
dc.subject | CELL LINES | en_US |
dc.subject | DIFFERENTIATION | en_US |
dc.subject | PLASTICITY | en_US |
dc.subject | INTEGRATION | en_US |
dc.title | Highly Pure and Expandable PSA-NCAM-Positive Neural Precursors from Human ESC and iPSC-Derived Neural Rosettes | en_US |
dc.type | Article | en_US |
dc.relation.no | 7 | - |
dc.relation.volume | 7 | - |
dc.identifier.doi | 10.1371/journal.pone.0039715 | - |
dc.relation.page | - | - |
dc.relation.journal | PLOS ONE | - |
dc.contributor.googleauthor | Kim, Dae-Sung | - |
dc.contributor.googleauthor | Lee, Dongjin | - |
dc.contributor.googleauthor | Kim, Han-Soo | - |
dc.contributor.googleauthor | Yoo, Jeong-Eun | - |
dc.contributor.googleauthor | Jung, Sung Jun | - |
dc.contributor.googleauthor | Lim, Bo Young | - |
dc.contributor.googleauthor | Jang, Jiho | - |
dc.contributor.googleauthor | Kang, Hoon-Chul | - |
dc.contributor.googleauthor | You, Seungkwon | - |
dc.contributor.googleauthor | Hwang, Dong-Youn | - |
dc.contributor.googleauthor | 김대성 | - |
dc.contributor.googleauthor | 이동진 | - |
dc.contributor.googleauthor | 김한수 | - |
dc.contributor.googleauthor | 유정은 | - |
dc.contributor.googleauthor | 정성준 | - |
dc.contributor.googleauthor | 임보영 | - |
dc.contributor.googleauthor | 장지호 | - |
dc.contributor.googleauthor | 강훈철 | - |
dc.contributor.googleauthor | 유승권 | - |
dc.contributor.googleauthor | 황동윤 | - |
dc.relation.code | 2012219766 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | eurijj | - |
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