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Multiparametric assessment of Cd2+ cytotoxicity using MTT-based microfluidic image cytometry

Title
Multiparametric assessment of Cd2+ cytotoxicity using MTT-based microfluidic image cytometry
Author
윤태현
Keywords
image cytometry; lab-on-a-chip; MTT assay; microfluidics; cytotoxicity; HIGH-THROUGHPUT; FLOW-CYTOMETRY; ASSAYS; DEVICE; CELLS
Issue Date
2012-08
Publisher
WILEY-BLACKWELL, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
Citation
Cytometry Part A, Vol. 81 A, No. 8, August 2012, Pages 691-697
Abstract
A modified MTT protocol-based microfluidic image cytometry (mu FIC) was performed to assess Cd2+ induced cytotoxicity. The expanded capabilities of mu FIC, such as in situ measurement, high-throughput, and multiparametric analysis of adherent cells under precisely controlled chemical environments of microfluidic channels, were demonstrated in this study. Multiparametric analysis of mu FIC data has enabled us to categorize the progress of cell death into at least four different subgroups based on their morphology and metabolic activity. These advantages of the MTT-based mu FIC as a simpler, cheaper, and faster in vitro cell-based assay tool have many implications in biomedical, pharmaceutical, toxicological, and biological application areas, and we propose this technique as a future high throughputhigh content screening (HT-HCS) platform for cytotoxicity assays and drug screening. (c) 2012 International Society for Advancement of Cytometry
URI
http://onlinelibrary.wiley.com/doi/10.1002/cyto.a.22079/fullhttp://hdl.handle.net/20.500.11754/41265
ISSN
1552-4922
DOI
10.1002/cyto.a.22079
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > CHEMISTRY(화학과) > Articles
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