Multiparametric assessment of Cd2+ cytotoxicity using MTT-based microfluidic image cytometry
- Title
- Multiparametric assessment of Cd2+ cytotoxicity using MTT-based microfluidic image cytometry
- Author
- 윤태현
- Keywords
- image cytometry; lab-on-a-chip; MTT assay; microfluidics; cytotoxicity; HIGH-THROUGHPUT; FLOW-CYTOMETRY; ASSAYS; DEVICE; CELLS
- Issue Date
- 2012-08
- Publisher
- WILEY-BLACKWELL, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
- Citation
- Cytometry Part A, Vol. 81 A, No. 8, August 2012, Pages 691-697
- Abstract
- A modified MTT protocol-based microfluidic image cytometry (mu FIC) was performed to assess Cd2+ induced cytotoxicity. The expanded capabilities of mu FIC, such as in situ measurement, high-throughput, and multiparametric analysis of adherent cells under precisely controlled chemical environments of microfluidic channels, were demonstrated in this study. Multiparametric analysis of mu FIC data has enabled us to categorize the progress of cell death into at least four different subgroups based on their morphology and metabolic activity. These advantages of the MTT-based mu FIC as a simpler, cheaper, and faster in vitro cell-based assay tool have many implications in biomedical, pharmaceutical, toxicological, and biological application areas, and we propose this technique as a future high throughputhigh content screening (HT-HCS) platform for cytotoxicity assays and drug screening. (c) 2012 International Society for Advancement of Cytometry
- URI
- http://onlinelibrary.wiley.com/doi/10.1002/cyto.a.22079/fullhttp://hdl.handle.net/20.500.11754/41265
- ISSN
- 1552-4922
- DOI
- 10.1002/cyto.a.22079
- Appears in Collections:
- COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > CHEMISTRY(화학과) > Articles
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