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Quantifying specific cell-polymer interactions using fluorescence correlation spectroscopy

Title
Quantifying specific cell-polymer interactions using fluorescence correlation spectroscopy
Author
이근용
Keywords
HYDROGELS; ADHESION; SURFACE; FIBROBLASTS; STIFFNESS; RGD
Issue Date
2011-05
Publisher
ROYAL SOC CHEMISTRY, THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND
Citation
SOFT MATTER 2011, 7 (10), 4876-4880
Abstract
Evaluation of interactions between polymers and cells is very important in many biomedical applications, including tissue engineering, as polymer scaffolds represent the extracellular matrix in the body and provide cells with three-dimensional framework. Alginate was modified with a peptide with the sequence of arginine-glycine-aspartic acid (RGD) to prepare cell-interactive polymers, and specific interactions between mouse fibroblasts and RGD peptide-modified alginates were investigated using fluorescence correlation spectroscopy (FCS). FCS was useful for determining cell-polymer interactions in a quantitative manner at nanomolar polymer concentrations, and the results were consistent with those obtained from the fluorescence resonance energy transfer (FRET) technique. This approach of quantitatively determining interactions between cells and polymers could be useful for developing novel polymer scaffolds for tissue engineering, as well as for designing highly efficient drug delivery systems.
URI
http://pubs.rsc.org/en/Content/ArticleLanding/2011/SM/c0sm01553a#!divAbstract
ISSN
1744-683X
DOI
10.1039/c0sm01553a
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
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