Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 윤채옥 | - |
dc.date.accessioned | 2018-02-27T01:34:48Z | - |
dc.date.available | 2018-02-27T01:34:48Z | - |
dc.date.issued | 2016-03 | - |
dc.identifier.citation | INTERNATIONAL JOURNAL OF DERMATOLOGY, v. 55, NO 3, Page. 356-361 | en_US |
dc.identifier.issn | 0011-9059 | - |
dc.identifier.issn | 1365-4632 | - |
dc.identifier.uri | http://onlinelibrary.wiley.com/doi/10.1111/ijd.12965/abstract?systemMessage=Please+be+advised+that+we+experienced+an+unexpected+issue+that+occurred+on+Saturday+and+Sunday+January+20th+and+21st+that+caused+the+site+to+be+down+for+an+extended+period+of+time+and+affected+the+ability+of+users+to+access+content+on+Wiley+Online+Library.+This+issue+has+now+been+fully+resolved.++We+apologize+for+any+inconvenience+this+may+have+caused+and+are+working+to+ensure+that+we+can+alert+you+immediately+of+any+unplanned+periods+of+downtime+or+disruption+in+the+future. | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/40667 | - |
dc.description.abstract | BackgroundKeloids are marked by an overabundance of extracellular matrix. The antifibrotic effect of hepatocyte growth factor (HGF) is achieved by increasing the expression of matrix metalloproteinases (MMPs) that drive extracellular matrix catabolism. As such, we cultivated an RGD-modified HGF-expressing adenovirus (dE1-RGD/lacZ/HGF) for introduction into keloid fibroblasts (KFs), looking at the subsequent impact on MMP-1 expression. MethodsKFs infected with either test virus as experimental group (dE1-RGD/lacZ/HGF) or its counterpart (dE1-RGD/lacZ) as control group were examined for HGF protein expression using an enzyme-linked immunosorbent assay (ELISA). Collagen (types I and III) and MMP-1 mRNA levels were also determined by reverse transcriptase-polymerase chain reaction, and ELISA was used to monitor MMP-1 protein expression. ResultsIn KFs harboring the test virus, high levels of HGF were induced at a multiplicity of infection ratio of 50 (3260.6162.7pg/ml) after 72hours of incubation. Furthermore, reverse transcriptase-polymerase chain reaction and ELISA confirmed that MMP-1 mRNA and protein expression rose significantly in KFs after transduction by the test virus (P˂0.05). However, mRNA levels of collagen were unaffected by the experimental group. ConclusionThese results suggest that an HGF-expressing adenovirus may be therapeutic for keloids by increasing MMP-1 expression. | en_US |
dc.description.sponsorship | This work was supported by grants from the National Research Foundation of Korea (NRF) grant funded by the Korean government (MEST) (NRF-2014R1A2A1A1105 1295, NRF-2009-0073055, W.J.L.). | en_US |
dc.language.iso | en | en_US |
dc.publisher | WILEY-BLACKWELL | en_US |
dc.subject | GENE-THERAPY | en_US |
dc.subject | HYPERTROPHIC SCARS | en_US |
dc.subject | COLLAGEN-SYNTHESIS | en_US |
dc.subject | LIVER-CIRRHOSIS | en_US |
dc.subject | CUTANEOUS CELLS | en_US |
dc.subject | WOUNDS | en_US |
dc.subject | STRATEGIES | en_US |
dc.subject | PATHWAYS | en_US |
dc.subject | FIBROSIS | en_US |
dc.subject | EFFICACY | en_US |
dc.title | Hepatocyte growth factor-expressing adenovirus upregulates matrix metalloproteinase-1 expression in keloid fibroblasts | en_US |
dc.type | Article | en_US |
dc.relation.no | 3 | - |
dc.relation.volume | 55 | - |
dc.identifier.doi | 10.1111/ijd.12965 | - |
dc.relation.page | 356-361 | - |
dc.relation.journal | INTERNATIONAL JOURNAL OF DERMATOLOGY | - |
dc.contributor.googleauthor | Jeon, Yeo Reum | - |
dc.contributor.googleauthor | Ahn, Hyo Min | - |
dc.contributor.googleauthor | Choi, Il Kyu | - |
dc.contributor.googleauthor | Yun, Chae Ok | - |
dc.contributor.googleauthor | Rah, Dong Kyun | - |
dc.contributor.googleauthor | Lew, Dae Hyun | - |
dc.contributor.googleauthor | Lee, Won Jai | - |
dc.relation.code | 2016001012 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | chaeok | - |
dc.identifier.orcid | http://orcid.org/0000-0002-9466-4531 | - |
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