Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 한중수 | - |
dc.date.accessioned | 2018-02-23T01:23:47Z | - |
dc.date.available | 2018-02-23T01:23:47Z | - |
dc.date.issued | 2011-06 | - |
dc.identifier.citation | Biochemical journal, Vol.436 No.1 [2011], 181-191 | en_US |
dc.identifier.issn | 0264-6021 | - |
dc.identifier.issn | 1470-8728 | - |
dc.identifier.uri | http://www.biochemj.org/content/436/1/181 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/40265 | - |
dc.description.abstract | Decidualization is a biological and morphological process occurring in hES (human endometrial stromal) cells. Previously, we reported that PLD1 (phospholipase D1) plays an important role in cAMP-induced decidualization of hES cells. In the present study, we focused on how PLD1 expression is up-regulated during decidualization. Treatment with PKA (protein kinase A) inhibitors (Rp-cAMP or H89) or a Ras inhibitor (manumycin) partially inhibited PLD1 expression and decidua formation in response to cAMP treatment. Interestingly, dual inhibition of PKA and Ras completely inhibited PLD1 expression and cAMP-induced decidualization. These results suggest that PLD1 expression during decidualization is controlled additively by PKA and Ras. The use of inhibitors showed that extracellular-signal-regulated kinase, a downstream effector of Ras, was required for PLD activation and the morphological changes during decidualization, but not for the increase in PLD1 protein. Next, to investigate the regulator of the PLD1 gene at the transcriptional level, a promoter assay using deletion mutants of the PLD1 promoter was performed; the result indicated that PR (progesterone receptor) was a possible regulator of the PLD1 gene. In addition, chromatin immunoprecipitation assays on the PLD1 promoter identified PR as a transcription factor for PLD1 expression during 8-Br-cAMP-induced decidualization. Taken together, our findings demonstrate that PKA and Ras are novel regulators of PLD1 expression and also identify PR as a transcription factor for PLD1 expression during the decidualization of hES cells. | en_US |
dc.description.sponsorship | This work was supported by the Korea Healthcare Technology R&D Project, the Ministry for Health, Welfare and Family Affairs, Republic of Korea [grant number A084985], a National Research Foundation of Korea (NRF) grant funded by the Korean Government (Ministry of Education, Science and Technology; MEST) [grant number 2010-0029503], and partly by the research fund of Hanyang University [grant number HY-2006-C]. | en_US |
dc.language.iso | en | en_US |
dc.publisher | PORTLAND PRESS - LONDON | en_US |
dc.subject | Decidualization | en_US |
dc.subject | Human endometrial stromal cell (hES cell) | en_US |
dc.subject | Phospholipase D1 (PLD1) | en_US |
dc.subject | Progesterone receptor (PR) | en_US |
dc.subject | Protein kinase A (PKA) | en_US |
dc.subject | Ras | en_US |
dc.title | The progesterone receptor as a transcription factor regulates phospholipase D1 expression through independent activation of protein kinase A and Ras during 8-Br-cAMP-induced decidualization in human endometrial stromal cells | en_US |
dc.type | Article | en_US |
dc.relation.volume | 436 | - |
dc.identifier.doi | 10.1042/BJ20101614 | - |
dc.relation.page | 181-191 | - |
dc.relation.journal | BIOCHEMICAL JOURNAL | - |
dc.contributor.googleauthor | Cho, Ju Hwan | - |
dc.contributor.googleauthor | Yoon, Mee-Sup | - |
dc.contributor.googleauthor | Koo, Jun Bon | - |
dc.contributor.googleauthor | Lee, Ki-Sung | - |
dc.contributor.googleauthor | Lee, Jung Han | - |
dc.contributor.googleauthor | Han, Joong-Soo | - |
dc.contributor.googleauthor | Kim, Yong Seok | - |
dc.relation.code | 2011201239 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | jshan | - |
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