Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박현희 | - |
dc.date.accessioned | 2018-02-22T04:54:35Z | - |
dc.date.available | 2018-02-22T04:54:35Z | - |
dc.date.issued | 2012-02 | - |
dc.identifier.citation | Drug Development Research, 2012, 73(1), P.35-42, 8p. | en_US |
dc.identifier.issn | 0272-4391 | - |
dc.identifier.issn | 1098-2299 | - |
dc.identifier.uri | http://onlinelibrary.wiley.com/doi/10.1002/ddr.20447/abstract | - |
dc.description.abstract | We investigated the effect of hetastarch, used for the treatment of acute ischemic stroke, on neuronal cell damage by oxidative stress, a main pathogenic mechanism in ischemic stroke. Neuronally differentiated PC12 cells (nPC12 cells) were treated with varying concentrations of hetastarch and hydrogen peroxide (H2O2), and their viability was measured with a 3,(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and trypan blue staining. The effect of hetastarch on free radical production by H2O2 was evaluated using the fluorescent probe 2','-dichlorodihydrofluorescein diacetate (DCFH-DA) and by quantifying the amount of 2,5- and 2,3-dihydroxybenzoic acid (DHBA). Additionally, the expression levels of BAX, Bid, Bcl-2, Bcl-xL, cytosolic cytochrome c, and cleaved caspase-3 were examined using Western blot analysis. Following exposure to 100 mu M H2O2, the viability of nPC12 cells significantly decreased; however, cell viability increased with hetastarch treatment. Free radical production related to H2O2 exposure was significantly reduced after 100 mu M hetastarch treatment. The expression levels of BAX, Bid, cytosolic cytochrome c, and activated caspase-3 were reduced, whereas Bcl-2 and Bcl-xL levels increased in H2O2-injured nPC12 cells treated with 100 mu M hetastarch, as compared with nPC12 cells that were treated with only 100 mu M H2O2. These results demonstrate that hetastarch can reduce oxidative stress-induced neuronal cell death. | en_US |
dc.description.sponsorship | Grant sponsor: Research fund of Hanyang University; Grant number: HY-2010-MC. | en_US |
dc.language.iso | en | - |
dc.publisher | Wiley-Blackwell | en_US |
dc.subject | hetastarch | en_US |
dc.subject | neuroprotection | en_US |
dc.subject | oxidative stress | en_US |
dc.subject | free radical | en_US |
dc.subject | PC12 cells | en_US |
dc.subject | hydrogen peroxide | en_US |
dc.subject | ischemic stroke | en_US |
dc.title | Hetastarch reduces neuronal cell death caused by oxidative stress | en_US |
dc.type | Article | en_US |
dc.relation.volume | 73 | - |
dc.identifier.doi | 10.1002/ddr.20447 | - |
dc.relation.page | 35-42 | - |
dc.relation.journal | DRUG DEVELOPMENT RESEARCH | - |
dc.contributor.googleauthor | Lee, Kyu-Yong | - |
dc.contributor.googleauthor | Koh, Seong-Ho | - |
dc.contributor.googleauthor | Park, Hyun-Hee | - |
dc.contributor.googleauthor | Lee, Young Joo | - |
dc.contributor.googleauthor | Kim, Sangjae | - |
dc.contributor.googleauthor | 이규용 | - |
dc.contributor.googleauthor | 고성호 | - |
dc.contributor.googleauthor | 박현희 | - |
dc.contributor.googleauthor | 이영주 | - |
dc.contributor.googleauthor | 김상재 | - |
dc.relation.code | 2012202646 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | newdiaz | - |
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