Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김승현 | - |
dc.date.accessioned | 2018-02-22T04:22:30Z | - |
dc.date.available | 2018-02-22T04:22:30Z | - |
dc.date.issued | 2012-02 | - |
dc.identifier.citation | Biochemical and Biophysical Research Communication, Feb 2012, 418(2), P.359-365 | en_US |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0006291X12000496?via%3Dihub | - |
dc.description.abstract | Disease progression of amyotrophic lateral sclerosis (ALS) is partially mediated by the toxic microenvironment established by microglia. In the present study, we used SOD1G93A transgenic mice as an in vivo ALS model and replaced microglia expressing mutant SOD1 (mSOD1) with microglia expressing wild-type SOD1 (w/tSOD1) to modulate the toxic microenvironment. Stereotactic injection of Clodronate liposome, a selective toxin against the monocyte/macrophage system, into the fourth ventricle of the brains of 12-week-old asymptomatic ALS mice reduced the number of microglia effectively in the central nervous system. Subsequent bone marrow transplantation (BMT) with bone marrow cells (BMCs) expressing w/tSOD1 and GFP leads to replacement of the endogenous microglia of the ALS mice with microglia expressing w/tSOD1 and GFP. The expression of mSOD1 in the other neural cells was not influenced by the replacement procedures, and immunological side effects were not observed. The replacement of microglia significantly slowed disease progression and prolonged survival of the ALS mice compared with the ALS mice treated by stereotactic injection of PBS-liposome and BMT with BMCs expressing mSOD1 or w/tSOD1. These results suggest that replacement of microglia would improve the neural cell microenvironment, thereby slowing disease progression. The mechanisms and functional implications of this replacement require further elucidation. Crown Copyright (C) 2012 Published by Elsevier Inc. All rights reserved. | en_US |
dc.description.sponsorship | This study was supported by the Research Settlement Fund for new faculty of SNU. This work was supported by the grant for the Future-based Technology Development Program (2010-0020232) funded by the National Research Foundation of Korea (NRF). | en_US |
dc.language.iso | en | en_US |
dc.publisher | Elsevier Science | en_US |
dc.subject | Amyotrophic lateral sclerosis (ALS) | en_US |
dc.subject | SOD1(G93A) transgenic mice | en_US |
dc.subject | Bone marrow transplantation (BMT) | en_US |
dc.subject | Clodronate liposome; Microglia | en_US |
dc.title | Replacement of microglial cells using Clodronate liposome and bone marrow transplantation in the central nervous system of SOD1(G93A) transgenic mice as an in vivo model of amyotrophic lateral sclerosis | en_US |
dc.type | Article | en_US |
dc.relation.no | 2 | - |
dc.relation.volume | 418 | - |
dc.identifier.doi | 10.1016/j.bbrc.2012.01.026 | - |
dc.relation.page | 359-365 | - |
dc.relation.journal | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.contributor.googleauthor | Cha, Choong Ik | - |
dc.contributor.googleauthor | Lee, Jae Chul | - |
dc.contributor.googleauthor | Cho, Yu Jin | - |
dc.contributor.googleauthor | An, Jaeyeol | - |
dc.contributor.googleauthor | Joo, Kyeung Min | - |
dc.contributor.googleauthor | Seong, Jinsil | - |
dc.contributor.googleauthor | Kim, Seung Hyun | - |
dc.contributor.googleauthor | Lee, Se Jeong | - |
dc.contributor.googleauthor | Nam, Do-Hyun | - |
dc.contributor.googleauthor | 차중익 | - |
dc.contributor.googleauthor | 이재철 | - |
dc.contributor.googleauthor | 조유진 | - |
dc.contributor.googleauthor | 안재열 | - |
dc.contributor.googleauthor | 주경민 | - |
dc.contributor.googleauthor | 성진실 | - |
dc.contributor.googleauthor | 김승현 | - |
dc.contributor.googleauthor | 이세정 | - |
dc.contributor.googleauthor | 남도현 | - |
dc.relation.code | 2012201235 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | kimsh1 | - |
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