Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 황승용 | - |
dc.date.accessioned | 2018-02-19T05:47:46Z | - |
dc.date.available | 2018-02-19T05:47:46Z | - |
dc.date.issued | 2011-10 | - |
dc.identifier.citation | In Sensors & Actuators: B. Chemical 2011, 157(2),735-741 | en_US |
dc.identifier.issn | 0925-4005 | - |
dc.identifier.uri | http://www.sciencedirect.com/science/article/pii/S0925400511004175?via%3Dihub | - |
dc.description.abstract | A novel PDMS and glass-based microfluidic device consisting of a micromixer and microreactor for DNA ligation is described in this article. The new passive type planar micromixer is 10.33 mm long and composed of a straight channel integrated with nozzles and pillars, and the microreactor is composed of a serpentine channel. Mixing was enhanced by convective diffusion facilitated by the nozzles and pillars. The performance of the micromixer was analytically simulated and experimentally evaluated. The micromixer showed a good mixing efficiency of 87.7% at a 500 mu L/min flow rate (Re = 66.5). DNA ligation was successfully performed using the new microfluidic device, and ligation time was shortened from 4 h to 5 min. When used for on-chip ligation, this new micromixer offers advantages of disposability and portability. (C) 2011 Elsevier B.V. All rights reserved. | en_US |
dc.language.iso | en | en_US |
dc.publisher | ELSEVIER SCIENCE SA, PO BOX 564, 1001 LAUSANNE, SWITZERLAND | en_US |
dc.subject | DNA ligation | en_US |
dc.subject | Micromixer | en_US |
dc.subject | Micropillar | en_US |
dc.subject | Micronozzle | en_US |
dc.subject | Serpentine microreactor | en_US |
dc.subject | PDMS-glass microbiochip | en_US |
dc.subject | SERPENTINE MICROCHANNEL | en_US |
dc.subject | POINT MUTATIONS | en_US |
dc.subject | MIXER | en_US |
dc.subject | MICROPUMP | en_US |
dc.subject | SYSTEMS | en_US |
dc.subject | PCR | en_US |
dc.title | DNA ligation using a disposable microfluidic device combined with a micromixer and microchannel reactor | en_US |
dc.type | Article | en_US |
dc.relation.no | 2 | - |
dc.relation.volume | 157 | - |
dc.identifier.doi | 10.1016/j.snb.2011.05.016 | - |
dc.relation.page | 735-741 | - |
dc.relation.journal | SENSORS AND ACTUATORS B-CHEMICAL | - |
dc.contributor.googleauthor | Ko, Yong-Jun | - |
dc.contributor.googleauthor | Maeng, Joon-Ho | - |
dc.contributor.googleauthor | Ahn, Yoomin | - |
dc.contributor.googleauthor | Hwang, Seung Yong | - |
dc.relation.code | 2011208645 | - |
dc.sector.campus | S | - |
dc.sector.daehak | GRADUATE SCHOOL[S] | - |
dc.sector.department | DEPARTMENT OF BIONANOTECHNOLOGY | - |
dc.identifier.pid | syhwang | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.