65 0

Hyperpolarized Binding Pocket Nuclear Overhauser Effect for Determination of Competitive Ligand Binding

Title
Hyperpolarized Binding Pocket Nuclear Overhauser Effect for Determination of Competitive Ligand Binding
Author
이영복
Keywords
hyperpolarization; ligands; NMR spectroscopy; proteins
Issue Date
2012-04
Publisher
WILEY-V C H VERLAG GMBH, PO BOX 10 11 61, D-69451 WEINHEIM, GERMANY
Citation
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 51, 21, 5179-5182
Abstract
Protein?mediated polarization transfer : Ligands L1 and L2 that competitively bind to a protein are subject to indirect spin?polarization transfer through the binding site of the protein. If protons H L1 of one ligand are hyperpolarized by dynamic nuclear polarization (DNP, see picture), signal intensities in the NMR spectrum of the second ligand become enhanced. The relative build?up of signal of the second ligand yields information on its binding epitope.
URI
http://onlinelibrary.wiley.com/doi/10.1002/anie.201201003/abstract;jsessionid=DA604202EBFA59438994FCE7657ED10A.f04t03http://hdl.handle.net/20.500.11754/37926
ISSN
1433-7851
DOI
10.1002/anie.201201003
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE