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dc.contributor.authorJong Wook Hong-
dc.date.accessioned2018-02-15T19:31:37Z-
dc.date.available2018-02-15T19:31:37Z-
dc.date.issued2011-08-
dc.identifier.citationANALYTICAL CHEMISTRY, 권: 83, 호: 16, 페이지: 6148-6153en_US
dc.identifier.issn0003-2700-
dc.identifier.urihttp://pubs.acs.org/toc/ancham/83/16/?-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/37830-
dc.description.abstractWe demonstrate the accommodation of log-scale concentration gradients of inhibitors on a single microfluidic chip with a semidirec 0000005E t dilution capability of reagents for the determination of the half inhibitory concentration o 00000AEC r IC(50). The chip provides a unique tool for hosting a wide-range of concentration gradient for studies that require an equal distribution of measuring points on a logarithmic scale. Using Matrix metalloproteinase IX and three of its inhibitors, marimastat, batimastat, and CP471474, we evaluated the IC(50) of each inhibitor with a single experiment. The present work could be applied to the systematic study of biochemical binding and inhibition processes particularly in the field of mechanistic enzymology and the pharmaceutical industry.en_US
dc.description.sponsorshipJ.Y.Y. and S.J. contributed to this work equally. This research was partially supported by a grant from Marine Bioprocess Research Center of the Marine Bio 21 Project funded by the Ministry of Land, Transport and Maritime, Republic of Korea. We also acknowledge partial support from the National Institutes of Health (NIH Grant R01 008392). We thank Jing Dai and Kirn Cramer for their help during the preparation of the manuscript.en_US
dc.language.isoenen_US
dc.publisherAMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USAen_US
dc.subjectMATRIX-METALLOPROTEINASE INHIBITORSen_US
dc.subjectMICROFLUIDIC DEVICEen_US
dc.subjectKINETIC-PARAMETERSen_US
dc.subjectENZYME ASSAYSen_US
dc.subjectGENERATIONen_US
dc.subjectMICROCHIPen_US
dc.subjectGRADIENTen_US
dc.subjectREADERen_US
dc.subjectCELLSen_US
dc.titleLog-scale dose response of inhibitors on a chipen_US
dc.typeArticleen_US
dc.identifier.doi10.1021/ac201177g-
dc.relation.page6148-6153-
dc.relation.journalANALYTICAL CHEMISTRY-
dc.contributor.googleauthorKim, Se-Kwon-
dc.contributor.googleauthorJambovane, Sachin-
dc.contributor.googleauthorYun, Jae Young-
dc.contributor.googleauthorJambovane, Sachin-
dc.contributor.googleauthorHong, Jong Wook-
dc.contributor.googleauthorKim, Se-Kwon-
dc.contributor.googleauthorCho, Sung-Hak-
dc.contributor.googleauthorDuin, Evert C.-
dc.relation.code2011200599-
dc.sector.campusS-
dc.sector.daehakGRADUATE SCHOOL[S]-
dc.sector.departmentDEPARTMENT OF BIONANOTECHNOLOGY-
dc.identifier.pidjwh-
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GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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