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The distinctive roles of erythroid specific activator GATA-1 and NF-E2 in transcription of the human fetal gamma-globin genes

Title
The distinctive roles of erythroid specific activator GATA-1 and NF-E2 in transcription of the human fetal gamma-globin genes
Author
김철근
Keywords
LOCUS-CONTROL REGION; RNA-POLYMERASE-II; CHROMATIN-STRUCTURE; HISTONE ACETYLATION; QUANTITATIVE-ANALYSIS; HYPERSENSITIVE SITES; REGULATORY REGIONS; DELETION MUTATIONS; EXPRESSION; MOUSE
Issue Date
2011-05
Publisher
OXFORD UNIV PRESS, GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND
Citation
NUCLEIC ACIDS RESEARCH, 권: 39, 호: 16, 페이지: 6944-6955
Abstract
GATA-1 and NF-E2 are erythroid specific activators that bind to the beta-globin locus. To explore the roles of these activators in transcription of the human fetal stage specific gamma-globin genes, we reduced GATA-1 and p45/NF-E2 using shRNA in erythroid K562 cells. GATA-1 or p45/NF-E2 knockdown inhibited the transcription of the gamma-globin genes, hypersensitive site (HS) formation in the LCR and chromatin loop formation of the beta-globin locus, but histone acetylation across the locus was decreased only in the case of GATA-1 knockdown. In p45/NF-E2 knockdown cells, GATA-1 binding was maintained at the LCR HSs and gamma-globin promoter, but NF-E2 binding at the LCR HSs was reduced by GATA-1 knockdown regardless of the amount of p45/NF-E2 in K562 cells. These results indicate that histone acetylation is dependent on GATA-1 binding, but the binding of GATA-1 is not sufficient for the gamma-globin transcription, HS formation and chromatin loop formation and NF-E2 is required. This idea is supported by the distinctive binding pattern of CBP and Brg1 in the beta-globin locus. Furthermore GATA-1-dependent loop formation between HS5 and 3'HS1 suggests correlation between histone modifications and chromatin looping.
URI
http://eds.b.ebscohost.com/eds/detail/detail?vid=0&sid=f45619af-fa41-4836-95c0-24b424c9e5a1%40sessionmgr120&bdata=Jmxhbmc9a28mc2l0ZT1lZHMtbGl2ZQ%3d%3d#AN=000294556800016&db=edswschttps://academic.oup.com/nar/article/39/16/6944/2411224
ISSN
0305-1048
DOI
10.1093/nar/gkr253
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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