Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 박용수 | - |
dc.date.accessioned | 2018-02-14T04:40:02Z | - |
dc.date.available | 2018-02-14T04:40:02Z | - |
dc.date.issued | 2011-11 | - |
dc.identifier.citation | Diabetes - Metabolism: Research and Reviews, Nov 01, 2011 27(8):P802-808 | en_US |
dc.identifier.issn | 1520-7560 | - |
dc.identifier.uri | http://onlinelibrary.wiley.com/doi/10.1002/dmrr.1254/abstract? | - |
dc.description.abstract | Background Reactive oxygen species are considered an important cause of the death of pancreatic beta cells, thereby triggering the development of type 2 diabetes as well as failure of islet transplantation. The biological properties of metallothionein (MT) and superoxide dismutase (SOD) are likely to be related to their antioxidant and free-radical scavenging abilities, but their access across biological membranes is limited.Methods We investigated whether Tat-MT and Tat-SOD fusion protein could be introduced into islets by a novel protein transduction technology and protect them from oxidative damage. We used 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) and Annexin V/propidium iodide assays to analyse cell viability, and assessed expression of apoptosis marker proteins by Western blotting. We examined the protective effect of Tat-MT and Tat-SOD on the development of diabetes and on graft failure after syngeneic islet transplantation into Otsuka Long Evans Tokushima Fatty (OLETF) rats and Imprinting Control Region (ICR) mice, respectively.Results Tat-MT and Tat-SOD were successfully delivered into the rat islets, and reactive oxygen species, nitric oxide, glucolipotoxicity-induced cell death, cytokine injury, and DNA fragmentation due to ischaemia-reperfusion in pancreatic beta cells were significantly reduced. In addition Tat-MT and Tat-SOD treatment protected OLETF rats from developing diabetes, and enhanced the survival of antioxidant-treated islets transplanted into the renal capsules of diabetic mice.Conclusions Transduction of Tat-MT and Tat-SOD proteins offers a new strategy for protecting against the development of diabetes by relieving oxidative stress. Copyright (C) 2011 John Wiley & Sons, Ltd. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Wiley-Blackwell | en_US |
dc.subject | diabetes | en_US |
dc.subject | rejection | en_US |
dc.subject | Tat fusion protein | en_US |
dc.subject | antioxidant | en_US |
dc.subject | oxidative stress | en_US |
dc.title | The combination of metallothionein and superoxide dismutase protects pancreatic beta cells from oxidative damage | en_US |
dc.type | Article | en_US |
dc.relation.volume | 27 | - |
dc.identifier.doi | 10.1002/dmrr.1254 | - |
dc.relation.page | 802-808 | - |
dc.relation.journal | DIABETES-METABOLISM RESEARCH AND REVIEWS | - |
dc.contributor.googleauthor | Park, Leejin | - |
dc.contributor.googleauthor | Min, Dongsoo | - |
dc.contributor.googleauthor | Kim, Hyunok | - |
dc.contributor.googleauthor | Park, Jinseu | - |
dc.contributor.googleauthor | Choi, Sooyoung | - |
dc.contributor.googleauthor | Park, Yongsoo | - |
dc.relation.code | 2011202561 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | parkys | - |
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