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In vivo effects of UV radiation on multiple endpoints and expression profiles of DNA repair and heat shock protein (Hsp) genes in the cycloid copepod Paracyclopina nana

Title
In vivo effects of UV radiation on multiple endpoints and expression profiles of DNA repair and heat shock protein (Hsp) genes in the cycloid copepod Paracyclopina nana
Author
신경훈
Keywords
UV-radiation; Copepod Paracyclopina nana; Multiple endpoints; Stable isotope; Heat shock protein(Hsp); DNA repair; FATTY-ACID-COMPOSITION; B RADIATION; MARINE-PHYTOPLANKTON; TIGRIOPUS-JAPONICUS; OXIDATIVE STRESS; DAPHNIA-MAGNA; ULTRAVIOLET-RADIATION; INTERTIDAL COPEPOD; ANTIOXIDANT ENZYME; MOLECULAR-CLONING
Issue Date
2015-08
Publisher
ELSEVIER SCIENCE BV
Citation
AQUATIC TOXICOLOGY, v. 165, Page. 1-8
Abstract
To evaluate the effects of ultraviolet (UV) radiation on energy acquisition and consumption, the copepod Paracyclopina nana was irradiated with several doses (0-3 kJ/m(2)) of UV. After UV radiation, we measured the re-brooding success, growth pattern of newly hatched nauplii, ingestion rate, and assimilation of diet. In addition, we checked the modulated patterns of DNA repair and heat shock protein (hsp) chaperoning genes of P. nana. UV-B radiation induced a significant reduction (7-87%) of the re-brooding rate of ovigerous females, indicating that UV-induced egg sac damage is closely correlated with a reduction in the hatching rate of UV-irradiated ovigerous female offspring. Using chlorophyll a and stable carbon isotope incubation experiments, we found a dose-dependent decrease (P˂ 0.05) in food ingestion and the rate of assimilation to the body in response to UV radiation, implying that P. nana has an underlying ability to shift its balanced-energy status from growth and reproduction to DNA repair and adaptation. Also, expression of P. nana base excision repair (BER)-associated genes and hsp chaperoning genes was significantly increased in response to UV radiation in P. nana. These findings indicate that even 1 kJ/m(2) of UV radiation induces a reduction in reproduction and growth patterns, alters the physiological balance and inhibits the ability to cope with UV-induced damage in P. nana. (C) 2015 Elsevier B.V. All rights reserved.
URI
https://www.sciencedirect.com/science/article/pii/S0166445X1500140X?via%3Dihubhttp://hdl.handle.net/20.500.11754/37383
ISSN
0166-445X; 1879-1514
DOI
10.1016/j.aquatox.2015.05.002
Appears in Collections:
COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > MARINE SCIENCE AND CONVERGENCE ENGINEERING(해양융합공학과) > Articles
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